Objective measurement of patch test reactions due to dental metal compounds by measuring the erythema index and quantity of corneous moisture

采用二硝基氯苯迟发性超敏小鼠模型，用MEXAMETER MX18【〇！R】)和角质层含水量（Q.G.M.；用CORNEOMETER CM825【〇！R】测量）。耳部肿胀率与E.I.呈极显著正相关，而与Q.C.M.无显著正相关。因此，E.I.被用来客观评价斑贴试验反应。为了建立最佳的评估方法，我们测量了测量间隔和贴片应用对正常志愿者在斑贴试验反应中的脑电的影响。在使用金属化合物和胶带的部位，去除胶带后30分钟内的E.I.明显高于去除胶带后40分钟的E.I.。贴贴试验后第2、3、7天的E.I.显著高于贴贴试验前。我们得出结论，为了最有效地消除胶带贴敷对E.I.的影响，测试部位E.I.的标准偏差值（S.D.V）应优先于对照部位（相邻胶带贴敷部位和相邻正常皮肤）的E.I.。我们分别在贴片试验后第2、3、7天测量了金属过敏患者（n=10）、刺激性反应患者（n=10）和对照组（n=10）的E.I. S.D.V.。金属过敏患者的E.I. S.D.V.在检查的所有日子里都明显高于对照组。刺激反应组第2、3天的S.D.V.高于对照组，第7天的S.D.V.高于对照组。仅在第7天，金属过敏患者的S.V.D.高于刺激性反应患者。这些结果表明，通过连续测量，MEXAMETER可以客观地评估斑贴试验反应。

Using the mice delayed hypersensitivity to dinitrochlorobenzene model, we compared the ear swelling rate with erythema index (E.I. ; measured with MEXAMETER MX18【○!R】) and quantity of corneous moisture (Q.G.M. ; measured with CORNEOMETER CM825【○!R】). The ear swelling rate showed a highly significant positive correlation with E.I. but not with Q.C.M. Therefore, E.I was employed for the objective assessment of patch test reactions.To establish the optimal method for this assessment, we measured the effects of measurement intervals and tape application on E.I. during the patch test reactions in normal volunteers. At the site of metal compound and tape application, E.I. was significantly higher within 30 min after tape removal than after >40 min. E.I. was significantly higher on the 2nd,3rd, and 7th day post-patch test than before the patch test. We concluded that to eliminate the effects of tape application on E.I. most effectively, the standard deviation value (S.D.V) of E.I. at the test site should be preferred over E.I. at the control sites (sites of adjacent tape application and the adjacent normal skin).We measured the S.D.V. of E.I. in patients with metal allergy (n=10), in those with an irritant reaction (n=10), and in the control group (n=10) on the 2nd,3rd, and 7th day post-patch test. The S.D.V. of E.I. was significantly higher in patients with metal allergy than in the control group on all days examined. S.D.V. was higher in patients with irritant reactions than in the control group on the 2nd and 3rd day, but not on the 7th day. Only on the 7th day, patients with metal allergy had a higher S.V.D. than those with an irritant reaction. These results suggested that patch test reactions can be objectively assessed with MEXAMETER by employing consecutive measurements.