INHERITANCE OF GENETIC MATERIAL IN PROKARYOTES

原核生物遗传物质的遗传

• 批准号: 3467778
• 负责人: donald biek
• 金额: $ 10.6万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-01 至 1994-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3467778
• 关键词: DNA footprinting; DNA replication; Escherichia coli; alleles; autoradiography; bacterial genetics; cell cycle; cell growth regulation; chromosome aberrations; cytogenetics; fluorescence microscopy; fusion gene; gel electrophoresis; gene complementation; gene expression; genetic mapping; genetic markers; genetic regulation; molecular cloning; molecular genetics; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; plasmids; prokaryote; replicon

The long-term objective of this proposed research is to understanding the mechanisms involved in the inheritance of DNA in prokaryotes. Maintenance of DNA molecules in growing populations of cells requires that the DNA be replicated and that the DNA molecules be distributed (partitioned) such that each daughter cell receives at least one copy of the replicon. The study of extrachromosomal replicons known as plasmids has been very important in our current understanding of these processes. Many plasmids-encode antibiotic resistance plasmids resistance genes and the role in the ability to treat patients in hospitals plays an important suffering from bacterial infection. A better understanding of the mechanisms involved in plasmid maintenance would potentially benefit an approach to their control in clinical settings. In addition, many important pharmaceutical products are made using plasmid-containing bacteria, and the stability of plasmids in such situations is important. Also, several viruses associated with human diseases are know to exist in a plasmid-like state in cells. Plasmid loci involved in plasmid maintenance in prokaryotes have been identified and characterized previously; however, knowledge of the interactions of plasmid components with cellular products is very limited. I propose to study partitioning of low copy number plasmids in E. coli, with the specific aim of identifying and characterizing plasmid interactions and how mechanistically they accomplish partitioning: Chromosomal mutants affected in partitioning of the low copy-number replicons F, Pl, pSClOl, or the chromosome will be isolated using a variety of genetic selections including effects on plasmid stability and extragenic suppression of plasmid oar mutations. The mutants will be analyzed using genetic and molecular techniques to determine what functions are affected and to begin to understand the roles they play in partitioning.

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