INHERITANCE OF GENETIC MATERIAL IN PROKARYOTES

原核生物遗传物质的遗传

• 批准号: 2181635
• 负责人: donald biek
• 金额: $ 11.24万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-01 至 1995-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2181635
• 关键词: DNA footprinting; DNA replication; Escherichia coli; alleles; autoradiography; bacterial genetics; cell cycle; cell growth regulation; chromosome aberrations; cytogenetics; fluorescence microscopy; fusion gene; gel electrophoresis; gene complementation; gene expression; genetic mapping; genetic markers; genetic regulation; molecular cloning; molecular genetics; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; plasmids; prokaryote; replicon

The long-term objective of this proposed research is to understanding the mechanisms involved in the inheritance of DNA in prokaryotes. Maintenance of DNA molecules in growing populations of cells requires that the DNA be replicated and that the DNA molecules be distributed (partitioned) such that each daughter cell receives at least one copy of the replicon. The study of extrachromosomal replicons known as plasmids has been very important in our current understanding of these processes. Many plasmids-encode antibiotic resistance plasmids resistance genes and the role in the ability to treat patients in hospitals plays an important suffering from bacterial infection. A better understanding of the mechanisms involved in plasmid maintenance would potentially benefit an approach to their control in clinical settings. In addition, many important pharmaceutical products are made using plasmid-containing bacteria, and the stability of plasmids in such situations is important. Also, several viruses associated with human diseases are know to exist in a plasmid-like state in cells. Plasmid loci involved in plasmid maintenance in prokaryotes have been identified and characterized previously; however, knowledge of the interactions of plasmid components with cellular products is very limited. I propose to study partitioning of low copy number plasmids in E. coli, with the specific aim of identifying and characterizing plasmid interactions and how mechanistically they accomplish partitioning: Chromosomal mutants affected in partitioning of the low copy-number replicons F, Pl, pSClOl, or the chromosome will be isolated using a variety of genetic selections including effects on plasmid stability and extragenic suppression of plasmid oar mutations. The mutants will be analyzed using genetic and molecular techniques to determine what functions are affected and to begin to understand the roles they play in partitioning.

这项拟议研究的长期目标是 了解DNA遗传的机制 原核生物 在不断增长的种群中维持DNA分子 需要DNA被复制， 分子被分布（分区），使得每个子细胞 接收复制子的至少一个拷贝。 研究 被称为质粒的染色体外复制子 对我们目前对这些过程的理解很重要。 许多质粒编码抗生素抗性质粒抗性 基因和在医院治疗病人的能力中的作用 在细菌感染中扮演着重要的角色 感染 更好地了解参与的机制， 质粒维持将潜在地有益于一种方法， 在临床环境中的控制。 此外，许多重要 使用含质粒的细菌制备药物产品， 并且在这种情况下质粒的稳定性是重要的。 此外，已知与人类疾病相关的几种病毒 在细胞中以类质粒的状态存在。 原核生物中参与质粒维持的质粒基因座 以前已经确定和表征;然而，知识 质粒成分与细胞产物的相互作用 是非常有限的 我建议研究低拷贝的划分 E.大肠杆菌，具体目的是鉴定 并描述了质粒的相互作用， 它们完成了分配： 低拷贝数复制子F、P1、pSC 101或pSC 102的分配 将使用多种遗传学方法分离染色体， 选择包括对质粒稳定性和基因外 抑制质粒OAR突变。 我们会分析变种人 利用遗传和分子技术来确定 并开始了解他们在 分区