PROTEIN KINASE C OF THE CORPUS LUTEUM

这项研究的目的是为了更好地了解激素 灵长类动物黄体功能的控制。 建议的研究 本文旨在研究蛋白激酶C的作用。 磷酸肌醇信号转导的一个重要组成部分 系统，在灵长类动物黄体的激素控制。 在 从实际意义上讲，从这些研究中获得的知识将 增加我们对月经周期调节的理解 增强我们调节生育和不育的能力， 妇女 本提案的具体目标是：1）开发检测试剂盒， 检查恒河猴蛋白激酶C的激素刺激 猴黄体; 2.）以确定是否激素， 已知在灵长类动物黄体的控制中起作用， 以及被认为具有这种作用的激素， 刺激（或抑制）恒河猴体蛋白激酶C 黄体;和，3.）为了将这些激素对 蛋白激酶C活性与细胞功能的关系 黄体在整个黄体期。 将开发三种测定法来检查激素激活 蛋白激酶C在灵长类动物黄体中的作用。 中的第一 这些将检查蛋白激酶C从 在激素刺激下，细胞质到膜部分。 的 二是利用聚丙烯酰胺凝胶电泳分析 蛋白激酶C磷酸化的内源性蛋白 荷尔蒙刺激。 第三次试验将涉及 蛋白激酶C在灵长类动物中的免疫组织化学定位 黄体 使用这些测定，激素LH和CG， 前列腺素F2 α、E2、I2和D2，催产素，阿片肽， 将评估儿茶酚胺和GnRH的刺激性或 抑制蛋白激酶C活性。 检测开发 激素研究最初将利用黄体中期的组织 相位 由于黄体是一个动态的组织， 在整个黄体期，酶反应 将在研究期间的不同时间进行评估。 黄体期与生理活动相关。

The objective of this research is to better understand the hormonal control of primate corpus luteum function. The studies proposed herein are designed to investigate the role of protein kinase C. an essential component of the phosphoinositide signal-transduction system, in the hormonal control of the primate corpus luteum. In a practical sense, the knowledge gained from these studies will increase our understanding of the regulation of the menstrual cycle and enhance our ability to modulate fertility and infertility in women. The specific aims of this proposal are: 1.) To develop assays to examine hormonal stimulation of protein kinase C in the rhesus monkey corpus luteum; 2.) To determine whether hormones which are known to play a role in the control of the primate corpus luteum, and hormones for which such a role has been suggested, are able to stimulate (or inhibit) protein kinase C in the rhesus monkey corpus luteum; and, 3.) To correlate the effects of these hormones on protein kinase C activity with the functional capacity of the corpus luteum throughout the luteal phase. Three assays will be developed to examine the hormonal activation of protein kinase C in the primate corpus luteum. The first of these will examine the translocation of protein kinase C from the cytosol to the membrane fraction upon hormonal stimulation. The second will utilize polyacrylamide gel electrophoretic analysis of endogenous proteins phosphorylated by protein kinase C in response to hormonal stimulus. The third assay will involve immunohistochemical localization of protein kinase C in the primate corpus luteum. Using these assays, the hormones LH and CG, prostaglandins F2alpha, E2, I2, and D2, oxytocin, opioid peptides, catecholamines, and GnRH will be assessed for stimulatory or inhibitory effects on protein kinase C activity. Assay development and hormone studies will initially utilize tissues from midluteal phase. Since the corpus luteum is a dynamic tissue which changes markedly in function throughout the luteal phase, enzyme response to specific hormones will be assessed at various times during the luteal phase and correlated with physiological activity.