Genetics and Genomics Core

基因组学和DNA测序研究核心摘要 SD BRIN于2001年在USD建立了基因组学核心，随后是DNA测序和基因分型 核心（西部核心）在BHSU在2004年，以提供关键的基础设施，以支持研究和培训 教师和学生在整个SD进行研究需要基因组或遗传技术的需求。 这一合并核心的具体目标是：1）提供经验丰富的工作人员、基础设施和仪器， 支持SD BRIN研究人员通过Genomics Core访问微阵列和qPCR服务， USD和DNA（桑格和NextGen）测序、基因分型和一般遗传学服务， WestCore在BHSU; 2）实施使用尖端方法和新技术，包括TaqMan- 基于蛋白质检测，microRNA微阵列和NextGen测序的小基因组，小， 有针对性的转录组和宏基因组学; 3）与教师密切合作，解决独特的问题， 个人研究项目; 4）提供咨询、技术援助和数据分析援助; 5） 继续实施适当的质量保证程序; 6）对可持续发展进行需求评估 生物医学/生物技术研究人员;和7）继续促进K-12，本科教育推广 和研究生。南达科他州基因组和遗传服务的可用性对 推进SD BRIN的生物医学研究和培训目标。的创新做法 将DNA测序核心放在主要的本科院校（BHSU）对 PUI的研究文化和能力。这增加了BHSU本科生的数量 学生参与研究和进入生物医学研究的职业生涯，并允许BHSU增加一个 综合基因组学硕士学位课程。通过实现这些具体目标， 基因组学核心设施和WestCore将确保提供高质量、尖端和相关的 对教师和学生的研究和培训目标至关重要的基因组和遗传技术 网络广泛。通过评估SD BRIN教师的研究需求，核心可以促进合作 具有相似或互补的研究兴趣和/或专业知识的教师之间， 多学科生物医学研究。Genomics Core和WestCore的董事和工作人员努力 通过发展和加强各级学生的科学和技术知识， 介绍遗传学/基因组学讲习班、研讨会和非正式教育机会。整体 教育目标的预期成果是增加科学和技术知识， 在K-12学生之间的赞赏，并提高了各种基因组和遗传学的知识和技能， 技术在本科生和研究生中的应用。因此，研究和教育服务 由核心员工提供的支持SD BRIN的总体目标和目的的进步。

Genomics and DNA Sequencing Research Core Summary SD BRIN established the Genomics Core at USD in 2001, followed by the DNA Sequencing & Genotyping Core (WestCore) at BHSU in 2004 in order to provide critical infrastructure to support the research and training needs of faculty and students conducting research throughout SD needing genomic or genetic technologies. The specific aims of this combined core is to: 1) provide experienced staff, infrastructure and instrumentation to support SD BRIN investigators with access to microarray and qPCR services through the Genomics Core at USD, and DNA (Sanger and NextGen) sequencing, genotyping, and general genetics services through WestCore at BHSU; 2) implement the use of cutting edge methods and new technology including TaqMan- based protein detection, microRNA microarray, and NextGen sequencing for small genomes, small and targeted transcriptomes and metagenomics; 3) collaborate closely with faculty to solve problems unique to individual research projects; 4) provide consultation, technical assistance and assistance with data analysis; 5) continue to implement appropriate quality assurance procedures; 6) conduct a needs assessment of SD biomedical/biotechnology researchers; and 7) continue to foster educational outreach for K-12, undergraduate and graduate students. The availability of genomic and genetic services in South Dakota is crucial for the advancement of the biomedical research and training objectives of SD BRIN. The innovative approach of placing the DNA sequencing core at a primarily undergraduate institution (BHSU) has had significant impact on the research culture and capabilities at this PUI. This has increased the number of BHSU undergraduate students participating in research and going into biomedical research careers and allowed BHSU to add a program for a master’s degree in Integrative Genomics. Through fulfillment of these specific aims, the Genomics Core Facility and WestCore will ensure the availability of high quality, cutting edge, and relevant genomic and genetic technologies critical to the research and training objectives of faculty and students network wide. By assessing the research needs of SD BRIN faculty, the cores can facilitate collaboration between faculty with similar or complementary research interests and/or expertise leading to sustainable, multidisciplinary biomedical research. The directors and staff of the Genomics Core and WestCore strive to enhance the science and technology knowledge of students at all levels through the development and presentation of genetics/genomics workshops, seminars, and informal education opportunities. The overall expected outcomes of the educational goals are increased science and technology knowledge and appreciation among K-12 students, and enhanced knowledge and skills in various genomic and genetic technologies among undergraduate and graduate students. Thus the research and educational services provided by the core staff support the advancement of SD BRIN’s overall goals and objectives.