ANTI-INFLAMMATORY EFFECTS OF PARASYMPATHETIC DRUGS ON ENDOMETRIAL CELLS

副交感神经药物对子宫内膜细胞的抗炎作用

• 批准号: 8360625
• 负责人: KATHLEEN M EYSTER
• 金额: $ 0.25万
• 依托单位: UNIVERSITY OF SOUTH DAKOTA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360625
• 关键词: Agonist; Anti-Inflammatory Agents; Anti-inflammatory; Biomedical Research; Cells; Conditioned Culture Media; Disease; Endometrial; Endometrial Stromal Cell; Exhibits; Funding; Genes; Goals; Grant; Inflammatory; Inflammatory Response; Laboratories; Measures; Mediator of activation protein; Muscarinic Acetylcholine Receptor; National Center for Research Resources; Parasympathetic Nervous System; Pharmaceutical Preparations; Pilocarpine; Principal Investigator; Property; Research; Research Infrastructure; Resources; Source; South Dakota; Time; Tumor Necrosis Factor-alpha; United States National Institutes of Health; Work; cost; endometriosis; macrophage; prevent; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Endometriosis is an inflammatory disease, and cells of endometriosis explants exhibit invasive properties. Both the inflammatory response and the invasive response can be mimicked by treatments of endometrial stromal cells in culture. This project will examine the anti-inflammatory effects of parasympathetic nervous system mediators on endometrial stromal cells in culture. The laboratory has identified a signature of inflammatory genes expressed in THESC cells in response to treatment with macrophage conditioned medium. THESC cells will be treated with macrophage conditioned medium to trigger the inflammatory response in the absence and presence of the muscarinic receptor agonist, pilocarpine. The expression of inflammatory genes (such as tumor necrosis factor alpha) will be measured by real-time PCR. The goal of the study is to determine whether pilocarpine treatment can prevent or reduce the inflammatory response to endometrial stromal cells to macrophage conditioned medium. Liz Hoffman was supported by $4000 in ARRA funds during the summer of 2010 and the lab received $1000. An undergraduate fellow from DWU worked with Dr. Eyster during the summer of 2010.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 子宫内膜异位症是一种炎症性疾病，子宫内膜异位症的细胞表现出侵袭性。 炎症反应和侵袭性反应都可以通过处理培养的子宫内膜基质细胞来模拟。 该项目将研究副交感神经系统介质对培养的子宫内膜间质细胞的抗炎作用。 该实验室已经确定了一个标志性的炎症基因表达的THESC细胞在处理与巨噬细胞条件培养基。 将用巨噬细胞条件培养基处理THESC细胞，以在不存在和存在毒蕈碱受体激动剂毛果芸香碱的情况下触发炎症反应。 将通过实时PCR测量炎症基因（如肿瘤坏死因子α）的表达。 本研究的目的是确定毛果芸香碱治疗是否可以预防或减少子宫内膜间质细胞对巨噬细胞条件培养基的炎症反应。 2010年夏天，利兹霍夫曼得到了ARRA基金4000美元的支持，实验室收到了1000美元。 2010年夏天，一位来自DWU的本科生与Eyster博士一起工作。