PROTEIN KINASE C OF THE CORPUS LUTEUM

这项研究的目的是为了更好地了解荷尔蒙 灵长类黄体功能的控制。这项研究建议 在此，旨在研究蛋白激酶C的作用。 肌醇磷脂信号转导的重要组成部分 在灵长类黄体的荷尔蒙控制系统中。在……里面 从实际意义上讲，从这些研究中获得的知识将 增加我们对月经周期调节的理解 并增强我们调节生育和不孕的能力 女人。 这项建议的具体目的是：1.)发展化验方法，以 检测荷尔蒙对恒河猴蛋白激酶C的刺激作用 2.猴黄体；以确定哪些激素是 已知在控制灵长类黄体方面起作用， 以及被认为具有这种作用的荷尔蒙能够 刺激(或抑制)恒河猴体内的蛋白激酶C 黄体；以及3.)将这些荷尔蒙的影响联系起来 蛋白激酶C活性与细胞功能的关系 整个黄体期的黄体。 将开发三种检测方法来检测荷尔蒙的激活 蛋白激酶C在灵长类黄体中的含量。第一个 这些将检查蛋白激酶C的转位。 在荷尔蒙刺激下，胞浆向膜部分转化。这个 其次将利用聚丙烯酰胺凝胶电泳法进行分析。 被蛋白激酶C磷酸化的内源性蛋白 荷尔蒙刺激。第三个化验将涉及 蛋白激酶C在灵长类动物中的免疫组织化学定位 黄体。使用这些分析，荷尔蒙黄体生成素和黄体生成素 前列腺素F2a、E2、I2和D2，催产素，阿片肽， 儿茶酚胺和促性腺激素释放激素的刺激性或 对蛋白激酶C活性的抑制作用。化验进展 激素研究最初将利用黄体中期的组织 相位。因为黄体是一个动态变化的组织 在整个黄体期都有明显的功能，酶反应 将在不同的时间对特定的激素进行评估 黄体期与生理活动相关。

The objective of this research is to better understand the hormonal control of primate corpus luteum function. The studies proposed herein are designed to investigate the role of protein kinase C. an essential component of the phosphoinositide signal-transduction system, in the hormonal control of the primate corpus luteum. In a practical sense, the knowledge gained from these studies will increase our understanding of the regulation of the menstrual cycle and enhance our ability to modulate fertility and infertility in women. The specific aims of this proposal are: 1.) To develop assays to examine hormonal stimulation of protein kinase C in the rhesus monkey corpus luteum; 2.) To determine whether hormones which are known to play a role in the control of the primate corpus luteum, and hormones for which such a role has been suggested, are able to stimulate (or inhibit) protein kinase C in the rhesus monkey corpus luteum; and, 3.) To correlate the effects of these hormones on protein kinase C activity with the functional capacity of the corpus luteum throughout the luteal phase. Three assays will be developed to examine the hormonal activation of protein kinase C in the primate corpus luteum. The first of these will examine the translocation of protein kinase C from the cytosol to the membrane fraction upon hormonal stimulation. The second will utilize polyacrylamide gel electrophoretic analysis of endogenous proteins phosphorylated by protein kinase C in response to hormonal stimulus. The third assay will involve immunohistochemical localization of protein kinase C in the primate corpus luteum. Using these assays, the hormones LH and CG, prostaglandins F2alpha, E2, I2, and D2, oxytocin, opioid peptides, catecholamines, and GnRH will be assessed for stimulatory or inhibitory effects on protein kinase C activity. Assay development and hormone studies will initially utilize tissues from midluteal phase. Since the corpus luteum is a dynamic tissue which changes markedly in function throughout the luteal phase, enzyme response to specific hormones will be assessed at various times during the luteal phase and correlated with physiological activity.