Automation of 3D cell model assembly by additive printing

通过增材打印实现 3D 细胞模型组装的自动化

• 批准号: EP/M506849/1
• 负责人: Ricky Wildman
• 金额: $ 13.69万
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2014
• 资助国家: 英国
• 起止时间: 2014 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FM506849%2F1
• 关键词: Automation; 3D; cell; model; assembly

This project shall test the feasibility of automating the manufacture of advanced cell-based analysis platforms by additiveprinting of platform components. The analytical platforms shall be 3D cell cultures which enhance the function of residentcells. Additive printing shall deposit biocompatible materials consistently and efficiently in 3D architectures within multiwellculture plates compatible with medium-throughput analysis. 3D-printed structures designed to mimic tissue architectureshall be populated with stem cell-derived populations with tissue-specific functionality selected to match the printedarchitecture; the feasibility of printing cells directly into these structures shall be studied. For demonstration purposes, thepotential of automated additive printing shall be evaluated using hepatocytes derived from iPS progenitor cells anddeposited in liver-relevant 3D patterns, with model functionality being compared against competing systems and animalmodels based on drug-metabolising enzyme levels. The outcome shall be a pre-industrial demonstration of the potential toapply additive printing in the assembly of 3D cell-based assays used in drug discovery and other industrial processes. Thisdemonstration will create a springboard for further research and development aimed at automation of advanced cell-basedmodel assembly to industrial quality standards.

该项目将测试通过平台组件的增材打印自动化制造先进的基于细胞的分析平台的可行性。分析平台应为3D细胞培养物，可增强驻留细胞的功能。增材打印应在与中等通量分析兼容的多孔培养板内的3D架构中一致且有效地存款生物相容性材料。设计用于模拟组织结构的3D打印结构应使用具有组织特异性功能的干细胞衍生群体进行填充，以匹配打印结构;应研究将细胞直接打印到这些结构中的可行性。出于演示目的，应使用源自iPS祖细胞并以肝脏相关3D模式沉积的肝细胞评估自动添加剂打印的潜力，并将模型功能与基于药物代谢酶水平的竞争系统和动物模型进行比较。其成果将是一个工业前的示范，展示在药物发现和其他工业过程中使用的基于3D细胞的测定组装中应用增材打印的潜力。这一演示将为进一步的研究和开发创造一个跳板，旨在实现先进的基于单元的模型组装的自动化，以达到工业质量标准。