Video Liquid Transmission Electron Microscopy

视频液体透射电子显微镜

• 批准号: EP/N010906/1
• 负责人: Giuseppe Battaglia
• 金额: $ 4.78万
• 依托单位: University College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FN010906%2F1
• 关键词: Video; Liquid; Transmission; Electron; Microscopy

In science more than anywhere, seeing is believing. Since Galileo time and his invention of the telescope, Hook and van Leeuwenhoek and their contribution into developing the first optical microscope, scientists have always had the necessity to see objects whether these were living or not. Since these pioneering times, we have now been given a great collection of tools that allow the visualisation of matter almost imaging molecule by molecule. Transmission electron microscopy is possibly the most powerful and versatile of these tools and indeed contributed to a myriad of scientific discoveries across Chemistry, Physics, Biology and Medicine. Today, although the ultimate frontier of imaging is the ability to visualise matter when dispersed in a liquid and how this very unique environment affects molecular organisation. Among these liquid environment, water is the most critical as being the most important ingredient of life. Yet to date, no electron microscopy can be performed in a liquid sample as its functioning is associated with high vacuum conditions and hence no liquid can exist. However we have now created new materials that act as transparent holders of liquid samples to place them under an electron beam and thus image their content. This can indeed create a unique imaging platform that will allow the imaging of a large plethora of materials (including biologicals) in water (or any other liquid) without the need to remove the liquid and hence observe their structure and dynamic nature in its own environment. Moreover we propose here to exploit the liquid nature of the sample to create a combined approach where liquid samples can easily be injected into an integrated unit that will image them with resolution approaching ten times the size of the same water molecules as well as to analyse important changes such as size, structure, optical properties and chemical nature.

在科学界，眼见为实比任何地方都重要。自伽利略时代和他发明望远镜以来，胡克和货车列文虎克以及他们对发展第一台光学显微镜的贡献，科学家们一直有必要看到物体，无论这些物体是否有生命。自从这些开创性的时代以来，我们现在已经获得了大量的工具，这些工具可以让物质可视化，几乎是一个分子一个分子地成像。透射电子显微镜可能是这些工具中最强大、最通用的，确实为化学、物理、生物和医学领域的无数科学发现做出了贡献。今天，虽然成像的最终前沿是能够可视化物质在液体中分散的能力，以及这种非常独特的环境如何影响分子组织。在这些液体环境中，水是最关键的，因为它是生命最重要的组成部分。迄今为止，还没有电子显微镜可以在液体样品中进行，因为其功能与高真空条件相关，因此没有液体可以存在。然而，我们现在已经创造了新的材料，作为液体样品的透明支架，将它们放置在电子束下，从而对其内容物进行成像。这确实可以创建一个独特的成像平台，该平台将允许在水（或任何其他液体）中对大量材料（包括生物物质）进行成像，而无需移除液体，从而在其自身环境中观察它们的结构和动态性质。此外，我们在这里提出利用样品的液体性质来创建一种组合方法，其中液体样品可以容易地注入到集成单元中，该集成单元将以接近相同水分子大小的十倍的分辨率对它们进行成像，以及分析重要的变化，例如大小，结构，光学性质和化学性质。

项目成果

On the design of precision nanomedicines

精密纳米药物的设计

• DOI: 10.26434/chemrxiv.5647969
• 发表时间: 2019
• 作者: Battaglia G

Design principles for precision targeting

精确瞄准的设计原则

• DOI: 10.26434/chemrxiv.5647969.v2
• 发表时间: 2018
• 作者: Battaglia G

Cover Feature: Separating Extreme pH Gradients Using Amphiphilic Copolymer Membranes (ChemPhysChem 16/2018)

封面专题：使用两亲性共聚物膜分离极端 pH 梯度 (ChemPhysChem 16/2018)

• DOI: 10.1002/cphc.201800644
• 发表时间: 2018
• 期刊: ChemPhysChem
• 影响因子: 2.9
• 作者: Ruiz-Pérez L

Bottom-up Evolution from Disks to High-Genus Polymersomes

从盘到高属聚合物体的自下而上进化

• DOI: 10.26434/chemrxiv.6108467
• 发表时间: 2018
• 作者: Battaglia G

End-to-end image analysis pipeline for liquid-phase electron microscopy.

液相电子显微镜的端到端图像分析管道。

• DOI: 10.1111/jmi.12889
• 发表时间: 2020-09
• 期刊: Journal of microscopy
• 影响因子: 2
• 作者: Marchello G;DE Pace C;Duro-Castano A;Battaglia G;Ruiz-PÉrez L
• 通讯作者: Ruiz-PÉrez L