TIME INTEGRATED MEASUREMENT OF HORMONE AVAILABILITY

激素可用性的时间综合测量

• 批准号: 3503021
• 负责人: STEPHEN E WADE
• 金额: $ 4.98万
• 依托单位: HAMMERSMITH LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-15 至 1987-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3503021
• 关键词: clinical biomedical equipment; corticosterone; high performance liquid chromatography; implant; monitoring device; pharmacokinetics

Production and testing of a needle- implantable device for estimating long term tissue availability of the hormone corticosterone in rats is proposed. This work contributes to the long term goal of developing a similar device for measurement of the tissue availability of a broad range of compounds in both research and medical settings. The device operates on the diffusion- sink principle: a compartment is created in which the concentration of the compound of interest is maintained at a negligible level, by action of a binding protein with high affinity and specificity for that compound; consequently, the compound continuously diffuses into the device at a rate that depends on its availability in the immediate environment and the diffusive resistance offered by the walls of the device. By measuring the accumulation of a compound by a subcutaneously implanted device over a suitable time interval, a direct estimate of its tissue availability is obtained. Compactness and high capacity are gained by using concentrated binding proteins purified by affinity chromatography. Potential applications include measurement of time integrated cortisol availability in clinical psychiatry for differential diagnosis of endogenous depression or melancholia.

用于估计长时间的针植入式器械的生产和测试 大鼠中皮质酮激素的组织利用率术语为 提出了 这项工作有助于实现开发一个 用于测量宽范围组织可用性的类似装置 研究和医疗环境中的化合物。 该设备运行于 扩散-下沉原理：创造一个隔间， 目标化合物的浓度保持在可忽略的水平， 水平，通过高亲和力和特异性结合蛋白的作用 对于该化合物;因此，该化合物不断扩散到 该设备的速率取决于其即时可用性 环境和扩散阻力提供的墙壁的 设备. 通过皮下注射测量化合物的积累， 在适当的时间间隔内植入器械， 获得组织可用性。 获得了紧凑性和高容量 通过使用亲和纯化的浓缩结合蛋白 层析 潜在的应用包括测量时间 临床精神病学中皮质醇的综合利用度 诊断为内源性抑郁症或精神分裂症。