INTEGRATING ESTRADIOL MEASURE FOR FERTILITY MANAGEMENT

将雌二醇测量纳入生育管理

• 批准号: 3499617
• 负责人: STEPHEN E WADE
• 金额: $ 5万
• 依托单位: HAMMERSMITH LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-08-01 至 1989-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3499617
• 关键词: affinity chromatography; biosensor device; enzyme linked immunosorbent assay; estradiol; fertility; human tissue; luminescence; ovulation detection /prediction; saliva; steroid hormone metabolism

The proposed research will test the applicability of a new method for time-integrating measurement of the tissue availability of hormones, to the ovarian hormone estradiol. The new method uses an ultraminiature device, installed in the mouth of a subject, to continuously accumulate hormones from the saliva. The rate of accumulation at any moment directly reflects the availability o the hormone to tissue. The total accumulation over some time (1 to 10 h) reflects the time integral of hormone availability to tissue. This is advantageous since estradiol levels in the circulation and in the saliva fluctuate widely over short time intervals, and only 1 - 2% of estradiol in blood is actually available to tissue. The new method, if applicable to estradiol, would represent both the most direct and the least invasive way of determining the actual degree of tissue exposure to estradiol. Estradiol is a primary regulator of reproductive physiology, and current estradiol measurements are of some use in predicting the onset of the fertile period and the time ovulation, and predicting the outcome of fertility- enhancement procedures. A more reliable and convenient method for predicting the onset of the fertile period and the likelihood of successful conception and pregnancy would potentially find a wide market. The Phase I research specifically will extend the measurement method to estradiol, applying rigorous in vitro tests of the method under conditions approximating those of the intended use.

拟议的研究将测试一种新方法的适用性 对于组织可用性的时间积分测量， 荷尔蒙，到卵巢激素雌二醇。 新方法 使用超小型装置，安装在受试者的口中， 从唾液中不断积累激素。 率 任何时候的积累都直接反映了 将激素输送到组织中。 一段时间内的总累积量（1 至10 h）反映了激素可用性的时间积分， 组织. 这是有利的，因为雌二醇水平在 循环和唾液在短时间内波动很大 血液中只有1 - 2%的雌二醇实际上 可用于组织。 新方法，如果适用于雌二醇， 将代表最直接和最小侵入性的方式 确定组织暴露于雌二醇的实际程度。 雌激素是生殖生理的主要调节因子， 目前的雌二醇测量在预测 排卵期的开始和排卵的时间，并预测 提高生育力的手术结果。 一个更 一种可靠、方便的方法来预测 生育期和成功受孕的可能性， 怀孕可能会有很大的市场。 i期 研究具体将扩展测量方法， 雌二醇，应用严格的体外试验方法， 接近预期用途的条件。