PREPARATION OF SOLVENT-FREE PANCREATIC TISSUE EXTRACTS

无溶剂胰腺组织提取物的制备

• 批准号: 3495605
• 负责人: BARRY D SEARS
• 金额: $ 5万
• 依托单位: PGE TECHNOLOGY, INC.
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-08-01 至 1987-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3495605
• 关键词: biomedical equipment development; fats; pancreas; solvents; tissue /cell preparation; triglycerides

The preparation of pancreatic tissue extracts requires the removal of fats, primarily triglycerides from crude tissue. Such extracted pancreatic tissue is of paramount importance in treating cystic fibrosis patients who suffer from pancreatic insufficiency. In the past a variety of organic solvents have been used to extract fats from crude tissue. The goal of this application is to develop a new methodology using super critical carbon dioxide to remove the fats from crude tissue. Under super critical conditions, carbon dioxide will function as a "solvent" to remove triglycerides from crude tissue, but not leave any solvent residue. A successful extraction methodology should give a higher quality of extracted pancreatic tissue than is now possible. The goal of the Phase I study is to demonstrate that pancreatic tissue treated by super critical carbon dioxide retains equivalent, if not greater, enzymatic activity compared to present commercial products. Phase II studies would involve clinical trials with cystic fibrosis patients.

胰腺组织提取物的制备需要去除脂肪， 主要是来自粗组织的甘油三酯。 这样提取的胰腺 组织在治疗囊性纤维化患者中是至关重要的， 患有胰腺功能不全。 在过去的各种有机 已经使用溶剂从粗组织中提取脂肪。 的目标 本申请旨在开发一种新的方法， 二氧化碳来去除粗组织中的脂肪 在超临界条件下 在这种条件下，二氧化碳将作为一种“溶剂”， 从粗组织中提取甘油三酯，但不留下任何溶剂残留物。 一 成功的提取方法应该提供更高质量的提取物 胰腺组织比现在可能的。 I期研究的目标是 证明用超临界碳处理的胰腺组织 二氧化物保留了相当于，如果不是更大的酶活性相比， 目前的商业产品。 II期研究将涉及临床 囊性纤维化患者的临床试验。