CLONAL ANALYSIS OF HUMAN NEOPLASIA
人类肿瘤的克隆分析
基本信息
- 批准号:3482317
- 负责人:
- 金额:$ 19.24万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1983
- 资助国家:美国
- 起止时间:1983-08-01 至 1992-01-31
- 项目状态:已结题
- 来源:
- 关键词:B lymphocyte DNA methylation acute leukemia adenocarcinoma adenoma biological polymorphism carcinoma cell differentiation colon neoplasms cytogenetics endonuclease gene expression genetic library genetic manipulation granulocyte human genetic material tag human subject hypogammaglobulinemia molecular cloning molecular oncology neoplasm /cancer genetics neoplasm /cancer immunology neoplasm /cancer remission /regression neoplastic cell neoplastic transformation radioassay recombinant DNA sex chromosomes sex linked trait
项目摘要
A powerful new strategy for analyzing the clonal origin of human cell
populations has been developed. This strategy involves the use of
recombinant DNA probes that simultaneously detect restriction fragment
length polymorphisms and methylation patterns of X-chromosome genes. Our
long term goals are to use this strategy to study specific central issues
in neoplasia and X-linked diseases predisposing to neoplasia. The
immediate goals are to continue our studies in the following areas:
I. EXTENSIONOF CLONAL ANALYSIS Additional genes from the active
X-chromosome will be cloned to increase the number of polymorphic markers
that can be used for clonal analysis. This will allow the study of
virtually any female.
II. ANALYSIS OF SPECIFIC DISEASES
1. Colonic neoplasms. The clonal origin of colonic adenomas and carcinomas
will be defined to determine whether these neoplasms are derived from one
or many precursor cells.
2. Transitional cell carcinomas of the bladder. Investigations will be
performed to determine whether the multiple bladder lesions found in
patients with this disease are derived from one transformation event with
subsequent intravesicular spread or multiple independent transformation
events.
3. Leukemias. Our studies have shown that acute nonlymphocytic leukemia
cells differentiate to form mature granulocytes both during active disease
and during remission. Studies are proposed to define the incidence of this
differentiation, determine whether specific genetic changes are compatible
with differentiation, and elucidate correlations between the capacity to
differentiate and various clinical parameters.
4. X-linked diseases predisposing to neoplasia. Clonal analysis has the
capacity to identify female carriers of certain X-linked diseases
associated with neoplasia. The feasibility of using such analyses to
provide genetic counselling to families with these diseases will be
explored.
一种分析人类细胞克隆起源的新策略
人口得到了发展。 该战略涉及使用
同时检测限制性片段的重组DNA探针
X染色体基因的长度多态性和甲基化模式。 我们
长期目标是利用这一战略来研究具体的中心问题
在肿瘤和易诱发肿瘤的X连锁疾病中。 的
近期目标是继续在以下领域进行研究:
I. 克隆分析的扩展来自活性表达载体的额外基因
X染色体将被克隆以增加多态性标记的数量
可用于克隆分析。 这将有助于研究
几乎所有的女性。
二.特定疾病分析
1.结肠肿瘤。 结肠腺瘤和癌的克隆起源
将被定义,以确定这些肿瘤是否来自一个
或许多前体细胞。
2.膀胱移行细胞癌。 调查将
进行,以确定是否发现多个膀胱病变,
患有这种疾病的患者来自一个转化事件,
随后囊内扩散或多次独立转化
事件
3.白血病 我们的研究表明急性非淋巴细胞白血病
细胞分化形成成熟的粒细胞,
在缓解期间。 建议进行研究以确定这种情况的发生率
分化,确定特定的遗传变化是否兼容
与分化,并阐明能力之间的相关性,
区分和各种临床参数。
4.易发生肿瘤的X连锁疾病。 克隆分析具有
确定某些X连锁疾病女性携带者的能力
与肿瘤相关。 利用这种分析来
为患有这些疾病的家庭提供遗传咨询,
探讨了
项目成果
期刊论文数量(0)
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会议论文数量(0)
专利数量(0)
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