IMMUNOASSAYS FOR TAXOL

该项目的总体目标是生产选择性免疫分析。 能够区别检测紫杉醇及其同系物，以及 用于检测常见紫杉烷环结构的通用免疫分析。紫杉醇 目前在第二阶段临床试验中显示出希望。高效液相色谱检测方法的研究 紫杉醇是可用的，但相关化合物和代谢物的方法 没有上报。拟议的免疫分析将提供另一种选择 紫杉醇及其相关化合物的分析方法，可补充 临床研究中的高效液相色谱方法学。此外，免疫分析显示 紫杉醇的特异性将有助于鉴定紫杉醇的高产 短叶红豆杉品系。我们计划合成具有良好特性的紫杉醇 应能在兔和小鼠体内引起抗体反应的免疫原。这个 第一阶段研究的目标是证明 利用多克隆兔制备紫杉醇特异性免疫分析方法 抗体。 第二阶段的研究将集中在提纯紫杉醇的特异性分析和 相关单抗免疫分析试剂盒的研制 紫杉烷。

The overall goal of this project is to produce selective immunoassays capable of differentially detecting taxol and congeners of taxol, and a generic immunoassay for detecting the common taxane ring structure. Taxol is currently showing promise in Phase II clinical trials. HPLC methods for taxol are available but methods for related compounds and metabolites have not been reported. The proposed immunoassays would provide an alternative analytical method for taxol and related compounds, which could complement HPLC methodology in clinical studies. In addition, the immunoassays specific for taxol would be useful for identifying high taxol producing strains of Taxis Brevifola. We plan to synthesize well characterized taxol immunogens which should elicit antibody responses in rabbits and mice. The goal of Phase I research will be to demonstrate the feasibility of producing a taxol-specific immunoassay utilizing polyclonal rabbit antibodies. Phase II research will focus on refining the taxol-specific assay and on production of additional monoclonal antibody-based immunoassays for related taxanes.