IMMUNOASSAYS FOR SAXITOXINS

岩藻毒素是一种神经毒素，是最有效的毒素之一。 已知的非蛋白质毒素。有几千个案例 全世界每年都有一例麻风性贝类中毒- 死亡率接近10%。因为没有解药可以解决这些问题 中毒，避免污染物质是唯一的手段 对控制权的控制。目前公认的检测岩藻毒素的方法 其天然衍生物是通过小鼠生物测定的。备择 已经寻找了一些方法，但每种方法都有一个或多个重要意义 缺点。免疫分析似乎是最有希望的方法。 到目前为止。目前对免疫分析的限制是缺乏能力 检测萨克托毒素组中的所有毒素和问题 样品基质中各组分的干扰。方法 被建议用于产生多克隆抗体和单抗 这将使得能够检测到所有的岩藻毒素。第一阶段将 重点研究了两种不同新毒素半抗原的制备 以及小鼠或小鼠的免疫反应的演示 兔子。第二阶段将延长工作，并最终在 发展合适的非放射性免疫分析方法。在……里面 除了用于萨克托毒素免疫分析外，抗体 所生产的产品可能作为治疗剂具有其他用途 在麻痹性贝类中毒的情况下。

The saxitoxins are neurotoxins and are among the most potent non-protein toxins known. There are several thousand cases of paralytic shellfish poisoning worldwide each year with a case- fatality rate approaching 10%. Since there is no antidote for such poisoning, avoidance of contaminated material is the only means of control. The currently accepted method of detecting saxitoxin and its natural derivatives is by mouse bioassay. Alternative methods have been sought, but each has one or more significant drawback. Immunoassays appear to be the most promising method to date. Current limitations on immunoassays are lack of ability to detect all toxins in the saxitoxin group and problems with interference due to components in the sample matrix. Methods are proposed for generating polyclonal and monoclonal antibodies which will enable detection of all of the saxitoxins. Phase I will focus on the preparation of two different neosaxitoxin haptens and the demonstration of an immunological response in mice or rabbits. Phase II will extend the work and culminate in the development of suitable non-radioactive immunoassays. In addition to use in saxitoxin immunoassays, the antibodies produced may have additional applications as therapeutic agents in cases of paralytic shellfish poisoning.