TAXOL PURIFICATION BY MAB IMMUNOADSORBENTS

本一期工程旨在测试净化的可行性， 免疫亲和层析法测定紫杉醇。 使用该技术 用于纯化低水溶性低分子量化合物， 溶解度至今尚未报道。 紫杉醇特异性单克隆抗体将被选择用于进一步的研究。 基于它们对紫杉醇的亲和力和它们的能力的研究： (i)在含有10-20%有机溶剂的水性缓冲液中结合紫杉醇 (ii)为了在含有更高浓度紫杉醇的水性缓冲液中释放紫杉醇， 相同有机溶剂的浓度 选择的紫杉醇特异性单克隆抗体（mAb）将在 固定在合适的固相基质上。 增加 水性缓冲液中有机溶剂的浓度，以及高和低浓度 将评价pH通过以下方式破坏紫杉醇结合的能力： 抗体而不使mAb变性。 第一阶段的目标是 开发了能够分离紫杉醇和紫杉醇的实验室规模方法， 三尖杉宁碱

This Phase I project is designed to test the feasibility of purifying taxol by immunoaffinity chromatography. The use of this technique for purification of a low molecular weight compound with low aqueous solubility has not been reported to date. Taxol-specific monoclonal antibodies will be selected for further study on the basis of their affinity for taxol and their ability: (i) to bind taxol in aqueous buffer containing 10-20% organic solvent (ii) to release taxol in aqueous buffer containing higher concentrations of the same organic solvent Selected taxol-specific monoclonal antibodies (mAbs) will be immobilized on suitable solid phase matrices. Increasing concentrations of organic solvent in aqueous buffer, and high and low pH will be evaluated for their ability to disrupt binding of taxol by antibody without denaturing the mAb. The goal of Phase I is the development of a bench scale process capable of separating taxol and cephalomannine.