IMMUNOASSAYS FOR BRYOSTATINS

该项目的总体目标是开发用于 在多种生物制剂中检测苔藓抑素1及其类似物 矩阵 草苔虫抑制素在植物中表现出很强的抗肿瘤活性， 临床前研究，并计划进入I期临床试验 今年晚些时候 需要灵敏的分析方法来确定 这些研究中的适当给药方案和最大化产量 在大规模分离这些稀缺的天然产物的过程中。 在 此外，苔藓抑素免疫测定的可用性将有助于 基础研究的努力，以确定生物合成和实际来源的 这些有趣的化合物 我们计划合成具有良好特征的 苔藓抑素免疫原，其应在兔中引起抗体应答， 小鼠 第一阶段研究的目标是证明 利用多克隆兔产生苔藓抑素免疫测定 抗体的 第二阶段的研究将集中在完善这些分析， 产生另外的基于单克隆抗体的免疫测定， 具体应用的特殊性，并制定程序 测定各种生物基质中苔藓抑素的浓度。

The overall goals of this project are to develop immunoassays for the detection of bryostatin 1 and its analogues in a variety of biological matrices. The bryostatins have exhibited strong antineoplastic activity in preclinical studies and are scheduled to enter Phase I clinical trials later this year. Sensitive analytical methods are needed for establishing the proper dosing regimens in these studies and for maximizing yields during the large scale isolation of these scarce natural products. In addition, the availability of bryostatin immunoassays would facilitate basic research efforts to determine the biosynthesis and actual source of these interesting compounds. We plan to synthesize well characterized bryostatin immunogens which should elicit antibody responses in rabbits and mice. The goal of Phase I research will be to demonstrate the feasibility of producing bryostatin immunoassays utilizing polyclonal rabbit antibodies. Phase II research will focus on refining these assays, production of additional monoclonal antibody-based immunoassays of varying specificities for particular applications, and developing procedures for determining bryostatin concentrations in various biological matricies.