PROCESS FOR CLONING DIFFERENTIALLY EXPRESSED GENES

克隆差异表达基因的方法

• 批准号: 3498763
• 负责人: ROBERT C MIERENDORF
• 金额: $ 4.99万
• 依托单位: NOVAGEN, INC.
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1993-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3498763
• 关键词: RNA; autoradiography; binding proteins; biotin; cell differentiation; chromatography; complementary DNA; gel electrophoresis; gene expression; genetic library; genetic markers; method development; molecular cloning; molecular genetics; nucleic acid chemical synthesis; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; radiotracer; scintillation counter; solvent extraction; synthetic nucleotide

The proposed research would investigate the feasibility of a method for direct isolation and cloning of genes associated with states of disease, cell differentiation, or metabolic induction. Phase I studies will focus on steps in the process that currently limit the widespread use of substractive nucleic acid hybridization as a means of isolating the target genes. In Phase II this technology will be integrated and optimized to allow the cloning of any gene that is active in one cell type but not another.

拟议的研究将调查一种方法的可行性 直接分离和克隆与疾病状态相关的基因， 细胞分化，或代谢诱导。第一阶段研究将集中于 关于目前限制广泛使用的进程中的步骤 作为分离靶标的一种方法--减数核酸杂交 基因。在第二阶段，这项技术将被集成和优化，以 允许克隆在一种细胞类型中活跃但不能克隆的任何基因 又一个。