PROCESS FOR CLONING DIFFERENTIALLY EXPRESSED GENES
PROCESS FOR CLONING DIFFERENTIALLY EXPRESSED GENES
克隆差异表达基因的方法
批准号：
3498763
负责人：
ROBERT C MIERENDORF
金额：
$4.99万
依托单位：
依托单位国家：
美国
项目类别：
财政年份：
1992
资助国家：
美国
项目状态：
已结题
起止时间：
1992-09-01 至 1993-04-30
关键词：
RNA autoradiography binding proteins biotin cell differentiation chromatography complementary DNA gel electrophoresis gene expression genetic library genetic markers method development molecular cloning molecular genetics nucleic acid chemical synthesis nucleic acid hybridization nucleic acid probes nucleic acid sequence radiotracer scintillation counter solvent extraction synthetic nucleotide
中文摘要
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英文摘要
The proposed research would investigate the feasibility of a method for
direct isolation and cloning of genes associated with states of disease,
cell differentiation, or metabolic induction. Phase I studies will focus
on steps in the process that currently limit the widespread use of
substractive nucleic acid hybridization as a means of isolating the target
genes. In Phase II this technology will be integrated and optimized to
allow the cloning of any gene that is active in one cell type but not
another.
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