VALIDATION OF CAFFEINE/METHACETIN 13C BREATH TESTS

描述：(改编自申请人的摘要)。这项研究将 调查健康志愿者咖啡因和美沙西丁呼气试验 肝细胞色素P450-IA2检测方法的验证 活动。炭烤牛肉的作用及变化 蛋白质/碳水化合物比例对这些底物肝脏代谢的影响 将会被检查。基本假设是这些呼气测试 将准确地估计肝脏微粒体的活性。长期目标 是为了验证这些测试在药物试验中的使用。然后这些测试就可以 可用于测定受试者肝微粒体的活动量 参加这样的试验。有人建议，了解这些知识 饮食和环境因素对药物代谢的影响会导致 13C-咖啡因和13C-美沙西丁在控制膳食中的代谢 句号。 比值质谱学。

DESCRIPTION: (Adapted from the Applicant's Abstract). This study will investigate caffeine and methacetin breath tests in normal volunteers and validate the use of the tests for assessing hepatic cytochrome P450-IA2 activity. The effect of charcoal broiled beef and changes in protein/carbohydrate ratio on the hepatic metabolism of these substrates will be examined. The fundamental hypothesis is that these breath tests will accurately estimate hepatic microsomal activity. The long term goal is to validate these tests for use in drug trials. The tests could then be used to determine the amount of hepatic microsomal activity of subjects participating in such trials. It is suggested that knowledge of the effect of dietary and environmental factors on drug metabolism would lead to more accurate determinations about therapeutic dosages for new drugs. Studies are planned using normal healthy subjects to examine the metabolism of 13C-caffeine and 13C-methacetin during controlled dietary periods. Metabolism of the test compounds will be monitored by isotope ratio mass spectrometry.