VALIDATION OF CAFFEINE/METHACETIN 13C BREATH TESTS

咖啡因/美沙西丁 13C 呼吸测试的验证

• 批准号: 3496160
• 负责人: DAVID A WAGNER
• 金额: $ 4.66万
• 依托单位: METABOLIC SOLUTIONS, INC.
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-05-01 至 1992-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3496160
• 关键词: acetamides; breath tests; caffeine; carbon; carbon dioxide; cytochrome P450; diet; dietary carbohydrates; dietary proteins; drug metabolism; human subject; liver function; liver metabolism; mass spectrometry; method development; microsomes; nutrition related tag; protein structure function; stable isotope; stable isotope diagnosis

DESCRIPTION: (Adapted from the Applicant's Abstract). This study will investigate caffeine and methacetin breath tests in normal volunteers and validate the use of the tests for assessing hepatic cytochrome P450-IA2 activity. The effect of charcoal broiled beef and changes in protein/carbohydrate ratio on the hepatic metabolism of these substrates will be examined. The fundamental hypothesis is that these breath tests will accurately estimate hepatic microsomal activity. The long term goal is to validate these tests for use in drug trials. The tests could then be used to determine the amount of hepatic microsomal activity of subjects participating in such trials. It is suggested that knowledge of the effect of dietary and environmental factors on drug metabolism would lead to more accurate determinations about therapeutic dosages for new drugs. Studies are planned using normal healthy subjects to examine the metabolism of 13C-caffeine and 13C-methacetin during controlled dietary periods. Metabolism of the test compounds will be monitored by isotope ratio mass spectrometry.

描述：（改编自申请人摘要）。 本研究将 研究正常志愿者的咖啡因和美沙西丁呼吸试验， 验证用于评估肝细胞色素P450-IA 2的检测的使用 活动 木炭烤牛肉的效果和变化， 蛋白质/碳水化合物比例对这些底物的肝脏代谢的影响 将被审查。 基本假设是这些呼吸测试 将准确估计肝微粒体活性。 长期目标 是为了验证这些测试在药物试验中的有效性。 这些测试可以 用于测定受试者的肝微粒体活性量 参与这样的审判。 有人建议，知识的 饮食和环境因素对药物代谢的影响将导致 更准确地确定新药的治疗剂量。 计划使用正常健康受试者进行研究， 控制饮食期间13 C-咖啡因和13 C-美沙西丁的代谢 时期 将通过同位素监测供试化合物的代谢 比率质谱法