13 C - LABELED TRIOLEIN FOR DIAGNOSTIC BREATH TESTS

13 C - 用于诊断呼吸测试的标记三油酸甘油酯

• 批准号: 3507433
• 负责人: DAVID J KYLE
• 金额: $ 22.34万
• 依托单位: MARTEK BIOSCIENCE CORPORATION
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1993-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3507433
• 关键词: algae; breath tests; diagnosis design /evaluation; flow cytometry; gas chromatography; high performance liquid chromatography; hydrolysis; laboratory rat; mass spectrometry; mutant; nutrition disorder diagnosis; oils; oleate; plant extracts; postmortem; solvent extraction; stable isotope diagnosis; toxicology

The 14C-triolein breath test is a popular alternative to the classical 72- hr fecal fat collection for measuring fat malabsorption because of its simplicity, and the rapidity with which a diagnosis can be made. However, because of the concern for the long term effects of low-level radiation (especially when ingested), this test is generally not used in children or women of child-bearing age. The replacement of the radioactive carbon with its stable isotope (13C) and its detection using an isotope ratio mass spectrometer, has now made the triolein breath test available to any population of subjects. A principal limitation in its use today is the high cost of 13C-labeled triolein. We are proposing a biological approach to the synthesis of triolein which will result in an 18-fold increase in the content of the label. Perlabeled (100% incorporation) 13C-triolein will be produced by the autotrophic growth of a high-oleic oil producing strain of microalgae using 13CO2 as the sole carbon source. It is our hope that the technology developed in this grant will reduce the production costs and enhance the sensitivity of this compound such that a 10-fold reduction in cost per patient test can be realized. By reducing this test cost and eliminating the ingestion of radioactive compounds, the triolein breath test should be more readily available to the clinician for diagnostic uses in women and children.

14 C-三油酸甘油酯呼气测试是经典的72-三油酸甘油酯呼气测试的流行替代品。 hr粪便脂肪收集用于测量脂肪吸收不良，因为其 简单性，以及可以进行诊断的快速性。 然而，在这方面， 由于对低水平辐射的长期影响的关注 （特别是当摄入），这项测试一般不用于儿童或 育龄妇女。 放射性碳的替换 其稳定同位素（13 C）及其使用同位素比质量的检测 光谱仪，现在已经使三油酸甘油酯呼吸测试提供给任何 受试者人群。 今天使用它的一个主要限制是 13 C标记三油酸甘油酯成本高。 我们提出了一种生物方法来合成三油酸甘油酯， 将导致标签内容增加18倍。 全标记 (100 13 C-三油酸甘油酯将由自养的 使用13 CO2作为微藻的高油酸油生产菌株的生长， 唯一的碳源。 我们希望， 这笔赠款将降低生产成本，提高敏感性， 这种化合物使得每个患者测试的成本降低10倍， 实现了 通过降低该测试成本并消除 放射性化合物，三油酸甘油酯呼吸测试应该更容易 可供临床医生用于妇女和儿童的诊断。