TRANSGENIC RODENT AS AN ANIMAL MODEL FOR AIDS

转基因啮齿动物作为艾滋病动物模型

• 批准号: 3505276
• 负责人: VEMURI REDDY
• 金额: $ 4.96万
• 依托单位: TSI CORPORATION
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-01 至 1991-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3505276
• 关键词: AIDS; CD4 molecule; DNA; disease /disorder model; electrophoresis; embryo /fetus; genetic manipulation; genetic mapping; genetically modified animals; human immunodeficiency virus 1; immunocytochemistry; in situ hybridization; laboratory rabbit; model design /development; nucleic acid hybridization; polymerase chain reaction; tissue /cell culture; transfection

AIDS is a fatal disease caused by human immunodeficiency virus type 1 (HIV- 1). A big stumbling block to the development of effective vaccines and therapeutics for AIDS is the lack of suitable animal model. Therefore, there is an urgent need to search for a common laboratory animal to be developed as an AIDS model. Recently two groups, one led by Kindt, T. and the other by Filice, G. independently discovered that rabbits can be infected by HIV-1 experimentally. However, infection can only be achieved by intraperitoneal injection or injection of virus producing cells in addition to high titers of HIV-1. We propose to produce transgenic rabbits expressing human CD4 gene in tissue specific manners. Those rabbits could serve as a better AIDS model than non-transgenic ones. In phase I research we will prepare the 40 kb human CD4 gene and test it in cell cultures for functional expression and susceptibility of transfected cells to HIV-1 infection. If our theory proves to be correct, we will inject the 40 kb human CD4 gene into rabbit embryos in phase II research.

艾滋病是一种由人类免疫缺陷病毒1型（HIV-1）引起的致命疾病。 1）。 这是开发有效疫苗的一大绊脚石， 艾滋病的治疗方法缺乏合适的动物模型。 因此，我们认为， 迫切需要寻找一种普通的实验室动物， 作为艾滋病的模型。 最近，两个研究小组，一个由Kindt，T。和 Filice，G.独立发现兔子可以 实验性感染了HIV-1 然而，只有通过 通过腹膜内注射或注射病毒产生细胞， 除了高滴度的HIV-1。 我们建议生产转基因兔子 以组织特异性方式表达人CD 4基因。 那些兔子可以 作为一个比非转基因更好的艾滋病模型 在第一阶段的研究中 我们将制备40 kb的人CD 4基因，并在细胞培养物中进行测试， 转染细胞的功能表达和对HIV-1的易感性 感染 如果我们的理论被证明是正确的，我们将注入40 kb 在第二阶段研究中，将人CD 4基因植入兔胚胎。

项目成果

Developmental and tissue-specific expression of human CD4 in transgenic rabbits.

转基因兔中人 CD4 的发育和组织特异性表达。

• DOI: 10.1002/mrd.1080400405
• 发表时间: 1995
• 期刊: Molecular reproduction and development
• 影响因子: 2.5
• 作者: Snyder,BW;Vitale,J;Milos,P;Gosselin,J;Gillespie,F;Ebert,K;Hague,BF;Kindt,TJ;Wadsworth,S;Leibowitz,P
• 通讯作者: Leibowitz,P

Tissue-specific expression of human CD4 in transgenic mice.

转基因小鼠中人类 CD4 的组织特异性表达。

• DOI: 10.1128/mcb.13.5.2952-2958.1993
• 发表时间: 1993
• 期刊: Molecular and cellular biology
• 影响因子: 5.3
• 作者: Gillespie,FP;Doros,L;Vitale,J;Blackwell,C;Gosselin,J;Snyder,BW;Wadsworth,SC
• 通讯作者: Wadsworth,SC