HUMAN GROWTH HORMONE PRODUCED IN TRANSGENIC RABBIT MILK

转基因兔奶中产生的人类生长激素

• 批准号: 3508230
• 负责人: VEMURI REDDY
• 金额: $ 27.78万
• 依托单位: TSI CORPORATION
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-12-15 至 1993-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3508230
• 关键词: embryo /fetus; fusion gene; gene expression; genetic promoter element; genetically modified animals; laboratory mouse; laboratory rabbit; lactation; microinjections; milk; molecular cloning; nucleic acid sequence; protein engineering; somatotropin; transfection

Human growth hormone (hGH) is an important therapeutic being produced at high cost by recombinant methods. The Phase I of this SBIR has demonstrated the feasibility of producing this hormone in the milk of transgenic mice. The present aim is to produce hGH in the milk of transgenic rabbits which are easy and economical to handle. Transgenic rabbits will be screened for hGH expression in their milk samples. Rabbits producing high levels of the hormone will be propagated. Methods will be devised to maximize milk yield and purify hGH in large amounts. Accomplishment of the neurons unknown challenges will give us a clear understanding of the feasibility of using animals as bioreactors to produce other important therapeutics in a least expensive manner.

人生长激素（hGH）是一种重要的治疗药物， 重组方法成本高。 该SBIR的第一阶段 证明了在牛奶中生产这种激素的可行性， 转基因小鼠。 本发明的目的是在哺乳动物的乳汁中产生hGH， 转基因兔，其易于和经济地处理。 转基因 筛选兔乳汁样品中的hGH表达。 兔 产生高水平的激素将被繁殖。 方法将 旨在最大限度地提高产奶量和大量纯化hGH。 完成对神经元的未知挑战将给我们一个清晰的认识 了解使用动物作为生物反应器生产的可行性 其他重要的治疗方法。