PRODUCTION OF HUMAN SOLUBLE CD4 PROTEIN IN CHICKEN EGGS

鸡蛋中人溶性 CD4 蛋白的生产

• 批准号: 3489113
• 负责人: VEMURI REDDY
• 金额: $ 3.56万
• 依托单位: TSI CORPORATION
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-30 至 1990-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3489113
• 关键词: AIDS; AIDS therapy; CD4 molecule; alpharetrovirus; avian sarcoma virus; chick embryo; egg /ovum; egg food product; fusion gene; gene expression; genetic enhancer element; genetic promoter element; genetically modified animals; nucleic acid sequence; protein biosynthesis; transfection; transposon /insertion element

Soluble CD4 is considered to have a great potential in AIDS therapy including treatment and prevention of AIDS. In vitro studies have estimated that a large excess (1000:1) of soluble CD4 is needed to achieve a significant reduction in infectious particles. It is likely that an excess of soluble CD4 is needed constantly over prolonged periods during therapy in AIDS patients. Conventional methods of production (cell culture and bacteria) may not be suitable to produce soluble CD4 in large amounts in the least expensive way. We propose to produce soluble CD4 in transgenic chicken eggs using chicken ovalbumin promoter-enhancer signals fused to the coding sequences of soluble CD4 domain. Use of chicken eggs as bioreactors to produce soluble CD4 will be highly innovative and very inexpensive.

可溶性CD 4被认为在艾滋病治疗中具有巨大的潜力 包括艾滋病的治疗和预防。 体外研究 据估计，需要大量过量（1000：1）的可溶性CD 4才能达到 感染性颗粒的显著减少。 很可能是一个 长期需要过量的可溶性CD 4， 治疗艾滋病患者。 常规生产方法（细胞培养 和细菌）可能不适合大量产生可溶性CD 4 以最便宜的方式。 我们建议在细胞中产生可溶性CD 4。 使用鸡卵清蛋白启动子-增强子信号的转基因鸡蛋 与可溶性CD 4结构域的编码序列融合。 使用鸡蛋 作为生物反应器生产可溶性CD 4将是高度创新的， 便宜.