COLOR BASED AUTOMATED MICRONUCLEI ASSAY

基于颜色的自动化微核分析

• 批准号: 3496685
• 负责人: HARRY L LOATS
• 金额: $ 4.91万
• 依托单位: LOATS ASSOCIATES, INC.
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-07-01 至 1991-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3496685
• 关键词: animal tissue; biomedical equipment development; cell nucleus; chromosome aberrations; colorimetry; computer human interaction; computer program /software; computer system design /evaluation; erythroid stem cell; genetic disorder diagnosis; image enhancement; image processing; mathematical model; personal computers; toxicant screening

DESCRIPTION (Adapted from applicant's abstract): This SBIR is for the development of an automated color based micronuclei assay system based on results of a prototype MSDOS color analysis system. The system will automatically locate and count polychromatic erythrocytes (PCEs), micronucleated polychromatic erythrocytes (MPCEs) and normochromatic erythrocytes (NCEs). During Phase I, a number of critical variable parameters previously developed will be adapted into a user-friendly interactive program capable of rapidly selecting the appropriate parameters for each new slide preparation. These parameters include: the level of background strain; criteria for acceptable erythrocyte size range and roundness; and color discrimination. Color discrimination is the key element for differentiation. The system will be implemented on a Mac IIFX computer. It will include a capability to automatically process multiple slides into a report complete with statistical analysis. The Phase I final report will include the results of a test of the system. The test set-up and evaluation will be conducted by a consultant.

描述（改编自申请人摘要）：本SBIR适用于 开发基于颜色的自动化微核试验系统， MSDOS颜色分析系统原型的结果。 系统将 自动定位和计数嗜多染红细胞（PCE）， 微核嗜多染红细胞（MPCE）和正染红细胞 红细胞（NCE）。 在第一阶段，一些关键的可变参数，以前 开发将被改编成一个用户友好的互动程序， 快速为每张新幻灯片选择合适的参数 准备. 这些参数包括：背景应变水平; 可接受的红细胞大小范围和圆度的标准;以及颜色 歧视 颜色辨别力是 分化 该系统将在Mac IIFX计算机上实施。 它将包括一个 能够将多张幻灯片自动处理为完整的报告 进行统计分析。 第一阶段的最终报告将包括 系统测试的结果。 测试设置和评估将 由顾问进行。