COLOR BASED AUTOMATED MICRONUCLEI ASSAY

描述(改编自申请人的摘要)：本SBIR适用于 一种基于颜色的自动微核试验系统的研制 一个原型MSDOS颜色分析系统的结果。系统将 自动定位和计数多染红细胞(PCE)， 微核多染红细胞(MPCE)和常染红细胞 红细胞(NCEs)。 在第一阶段期间，之前的一些关键变量参数 开发的将被改编成用户友好的交互程序 为每张新幻灯片快速选择合适的参数 准备工作。这些参数包括：背景应变水平； 可接受的红细胞大小范围和圆度标准；以及颜色 歧视。颜色辨别力是 差异化。 该系统将在Mac IIFX计算机上实现。它将包括一个 自动将多张幻灯片处理成一份报告的功能完成 并进行统计分析。第一阶段最终报告将包括 该系统的测试结果。测试设置和评估将是 由一位顾问进行。

DESCRIPTION (Adapted from applicant's abstract): This SBIR is for the development of an automated color based micronuclei assay system based on results of a prototype MSDOS color analysis system. The system will automatically locate and count polychromatic erythrocytes (PCEs), micronucleated polychromatic erythrocytes (MPCEs) and normochromatic erythrocytes (NCEs). During Phase I, a number of critical variable parameters previously developed will be adapted into a user-friendly interactive program capable of rapidly selecting the appropriate parameters for each new slide preparation. These parameters include: the level of background strain; criteria for acceptable erythrocyte size range and roundness; and color discrimination. Color discrimination is the key element for differentiation. The system will be implemented on a Mac IIFX computer. It will include a capability to automatically process multiple slides into a report complete with statistical analysis. The Phase I final report will include the results of a test of the system. The test set-up and evaluation will be conducted by a consultant.