COLOR-BASED AUTOMATED MICRONUCLEUS SYSTEM

基于颜色的自动化微核系统

• 批准号: 2154741
• 负责人: HARRY L LOATS
• 金额: $ 24.54万
• 依托单位: LOATS ASSOCIATES, INC.
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-07-01 至 1995-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2154741
• 关键词: artificial intelligence; bioassay; biomedical automation; blood; bone marrow; colorimetry; computer data analysis; cytogenetics; erythrocytes; method development; micronucleus; mutagen testing

The overall objective of this Phase II application is the completion and validation of a color-based Micronuclei Assay System. Current federal plans include extensive use of the MNA to determine the potential genetic toxicity of various chemicals and drugs. The automation of MNA, based on color separation of polychromatic erythrocytes and normochromatic erythrocytes in bone marrow and peripheral blood preparations, employs innovative neural network techniques. A new 160-slide changer has been developed which allows total experiment operation without manual interruption, allowing a system to operate within and outside of normal working hours. Automation produces significantly improved statistics with significantly reduced manpower requirements. there is a large and building commercial requirement for these systems both in the US and Japan.

第二阶段申请的总体目标是完成和 基于颜色的微核测定系统的验证。 当前联邦 计划包括广泛使用MNA来确定潜在的遗传 各种化学品和药物的毒性。 多边核方案的自动化， 对嗜多染红细胞和正染红细胞的颜色分离 骨髓和外周血制备物中的红细胞，采用 创新的神经网络技术 一个新的160幻灯片转换器已经 开发了一种无需手动即可进行总实验操作的 中断，允许系统在正常范围内和正常范围外运行 工作时间 自动化显著改善了统计数据 大大减少了人力需求。 有一个很大的， 在美国和欧洲， 日本