DEVELOPMENT OF A HYBRID ARTIFICIAL ORGAN

本研究的目的是确定使用致密， 半渗透性聚合物膜用于免疫隔离体内的外来组织 杂交人造器官 将合成一系列聚合物， 其特征在于渗透性、细胞毒性和生物稳定性。 聚合物 组合物/形态将变化以产生一系列聚合物， 逐步增加渗透性。 将从溶液中铸膜 在均匀条件下使用实验室网涂布机涂布到剥离衬垫上 空气流动和热历史。 将使用相同的铸造溶液 制备用作细胞包的小直径中空纤维膜， 小鼠植入和体外细胞生长实验。 使用一系列 永久解决方案，平膜对水的渗透性， 葡萄糖和几种蛋白质将在非稳态下测量 扩散实验 将使用灵敏的量热/染色方法 为了定量葡萄糖和蛋白质， 选定永久性/膜的渗透系数计算 对. 在第二阶段，我们将建立和植入混合器官， 用于培养的和/或遗传的保护性的、选择性渗透的屏障 工程细胞 在第三阶段，第二阶段开发的膜将被 由我们的许可证持有人作为混合人工 机关

The goal of this study is to determine the feasibility of using a dense, semipermeable polymer membrane for immuno-isolation of foreign tissue in a hybrid artificial organ. A series of polymers will be synthesized and characterized for permeability, cytotoxicity and biostability. The polymer composition/morphology will be varied to yield a series of polymers with stepwise increasing permeability. Membranes will be cast from solution onto release liner, using a laboratory web coater under uniform conditions of air flow and thermal history. The same casting solution will be used to prepare small-diameter, hollow-fiber membranes for use as cell packets for mouse implant and in vitro cell growth experiments. Using a series of permanent solutions, the permeability of the flat membranes to water, glucose and several proteins will be measured in a non-steady-state diffusion experiment. Sensitive calorimetric/staining methods will be used to quantify glucose and proteins which permeate the membranes for calculation of permeability coefficients for selected permanent/membrane pairs. In Phase II we will build and implant hybrid organs with protective, selectively-permeable barriers for cultured and/or genetically engineered cells. In Phase III the membranes developed in Phase II will be offered commercially by our licensee as (components of) hybrid artificial organs.