Surface Modified Polymers for Tissue Engineering

描述（由申请人提供）： 本提案的目的是确定使用新型表面改性端基（SME）优化聚合物组织支架的可行性。将用五种不同的SME合成生物稳定的嵌段聚氨酯（SPU），并通过体外细胞培养进行测试。这些表面改性的SPU将包括三种疏水性降低的“被动”SME;氟碳化合物、硅酮和聚环氧乙烷（PEO），结合肝素的SME，和共价结合肽的PEO SME。基础聚合物将是Biospan SPU，这是一种广泛使用的心血管生物材料，具有全面的FDA主文件。将通过和频发生（SFG）振动光谱、原子力显微镜（AFM）和接触角测角法对表面进行表征。将在Instron上测量拉伸性能。第一阶段将确定细胞对材料水提取膜的反应。体外研究将包括：细胞粘附力、细胞迁移、细胞增殖、细胞凋亡和细胞分化。在第II阶段，将使用有前景的材料制造用于体内试验的小直径微孔血管移植物，使用PI已经为两种现有临床SPU血管移植物开发的制造方法。在第二阶段，中小企业将被耦合到一个基础聚合物，生物降解液体副产品：一个新的家庭聚氧化乙烯嵌段共聚物与定制的降解率。

DESCRIPTION (provided by applicant): The goal of this proposal is to determine the feasibility of optimizing polymeric tissue scaffolds using novel Surface Modifying Endgroups (SME). Biostable segmented polyurethane's (SPU) will be synthesized with five different SMEs and tested by in vitro cell culture. These surface-modified SPUs will include three 'passive' SMEs with decreasing hydrophobicity; fluorocarbon, silicone and polyethyleneoxide (PEO), an SME that binds heparin, and a PEO SME that covalently bind peptides. The base polymer will be Biospan SPU, a widely used cardiovascular biomaterial with a comprehensive FDA Master File. Surfaces will be characterized by Sum Frequency Generation (SFG) vibrational spectroscopy, Atomic Force Microscopy (AFM), and Contact Angle Goniometry. Tensile properties will be measured on an Instron. Phase I will determine cellular response to water-extracted films of the materials. In vitro studies will include: cell adhesion force, cell migration, cell proliferation, cell apoptosis, and cell differentiation. In Phase II, promising materials will be used to manufacture a small-diameter, microporous vascular graft for in vivo testing, using fabrication methods already developed by the PI for two existing clinical SPU vascular grafts. In Phase II SMEs will be coupled to a base polymer that biodegrades to liquid by-product: a novel family of polyethyleneoxide block copolymers with tailorable degradation rate.