MBRS

MBRS

• 批准号: 3513477
• 负责人: Sandra Bonetti
• 金额: $ 39.79万
• 依托单位: COLORADO STATE UNIVERSITY-PUEBLO
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-08-25 至 1997-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3513477
• 关键词: minority institution research support

This project proposes to examine the structures of low and high molecular weight mycelial glycopeptides from Penicillium charlesii and to determine whether these polymers serve as precursors and/or points of attachment or anchors for peptidophosphoglactomannans(pPGMs) or the extracellular glycohydrolases, phosphatases and proteases that are co-released with pPGMs. In order to establish the precursor/product relationships between the mycelial glycopeptides and the extracellular pPGMS, the structures of the cell wall, membrane, and extracellular pPGMs will be characterized. The structural relationships between the glycopeptides will be investigated by one and two dimensional NMR spectroscopy and GC/mass spectrometry in conjunction with amino acid analyses of peptide components. In addition, the biosynthetic relationships will be probed by using radiolabeled carbohydrate and peptide precursors and by biotinylation of cell walls, followed by isolation of the corresponding glycopeptides from the cell wall, membrane, and extracellular fractions. Establishing the anchor of these glycopeptides to the cell wall or membrane is important to the understanding of the physiological events leading to the release of pPGMs, hydrolytic enzymes, and potential allergens from the Penicillium charlesii.

该项目建议检查低分子和高分子的结构 重量来自charlesii的菌丝糖肽并确定 这些聚合物是否充当依恋的前体和/或 肽磷酸氨酰氨基甘脉（PPGM）或细胞外的锚定锚 甘脂性酒精酶，磷酸酶和蛋白酶，与之共同发行 ppgms。 为了建立前体/产品之间的关系 菌丝糖肽和细胞外PPGM，结构 细胞壁，膜和细胞外PPGM将被表征。 糖肽之间的结构关系将是 通过一维NMR光谱和GC/质量研究 光谱法与肽分析的肽分析 成分。 此外，生物合成关系将由 使用放射性标记的碳水化合物和肽前体，然后 细胞壁的生物素化，然后分离 细胞壁，膜和细胞外级分的糖肽。 将这些糖肽的锚定在细胞壁上或 膜对于理解生理事件很重要 导致PPGM，水解酶和电势的释放 charlesii的过敏原。