Ultrathin fluorescence microscope in a needle

超薄针状荧光显微镜

• 批准号: EP/R019274/1
• 负责人: Michael Hughes
• 金额: $ 12.84万
• 依托单位: University of Kent
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FR019274%2F1
• 关键词: Ultrathin; fluorescence; microscope; needle

Optical biopsy techniques allow high-resolution images of human tissue to be obtained in real time, without the need for invasive biopsy and histology. A common approach to optical biopsy is fluorescence endomicroscopy, whereby a flexible probe containing a fibre imaging bundle obtains video-rate images from fluorescently-labelled tissue. However, these probes only allow the most superficial tissue layers to be imaged. More recently, there has been interest in incorporating fluorescence microscopy probes into needles which could penetrate below the surface of organs, thus allowing deep-tissue imaging. However, there is currently a severe trade-off between the quality of the images (in terms of image size and resolution) and the diameter of the needle probe.In this project, a new approach for incorporating a microscope into a needle will be developed. Like conventional endomicroscopes, the needle will use a fibre imaging bundle to transmit images. However, in order to obtain higher quality images, the resolution limits of these bundles will be overcome. Conventionally, each fibre in a fibre bundle transmits only one 'pixel' of information. The new imaging system will exploit the fact that additional information can be encoded within the multiple modes of light propagation which each fibre core supports. Unlike most previous approaches which have sought to exploit fibre modes as information channels, no complex holographic reconstructions or wave-shaping using spatial light modulators will be required. Instead, an approach similar to single-pixel imaging will allow high-speed reconstruction of wide-field or optically-sectioned fluorescence images following a simple calibration procedure.

光学活检技术可以实时获得人体组织的高分辨率图像，而不需要侵入性活检和组织学。光学活检的常见方法是荧光内窥镜检查，其中包含光纤成像束的柔性探针从荧光标记的组织中获取视频速率图像。然而，这些探头只能对最表层的组织层进行成像。最近，人们对将荧光显微镜探针合并到可以穿透器官表面以下的针中产生了兴趣，从而可以进行深层组织成像。然而，目前图像质量（图像尺寸和分辨率）和针探针的直径之间存在严重的权衡。在该项目中，将开发一种将显微镜合并到针中的新方法。与传统的内窥镜一样，该针将使用光纤成像束来传输图像。然而，为了获得更高质量的图像，这些捆绑包的分辨率限制将被克服。传统上，光纤束中的每根光纤仅传输一个“像素”信息。新的成像系统将利用这样一个事实：附加信息可以在每个光纤芯支持的多种光传播模式中进行编码。与大多数先前试图利用光纤模式作为信息通道的方法不同，不需要使用空间光调制器进行复杂的全息重建或波形整形。相反，类似于单像素成像的方法将允许在简单的校准过程后高速重建宽视场或光学切片荧光图像。

项目成果

Reduced motion artifacts and speed improvements in enhanced line-scanning fiber bundle endomicroscopy.

• DOI: 10.1117/1.jbo.26.5.056501
• 发表时间: 2021-05
• 期刊: Journal of biomedical optics
• 影响因子: 3.5
• 作者: Thrapp A;Hughes M
• 通讯作者: Hughes M

Reduced motion artifacts and speed improvements in enhanced line-scanning fiber bundle endomicroscopy

增强型线扫描纤维束内窥镜检查中运动伪影的减少和速度的提高

• DOI: 10.48550/arxiv.2012.04492
• 发表时间: 2020
• 作者: Thrapp A

Motion compensation in structured illumination fluorescence endomicroscopy

结构照明荧光内窥镜中的运动补偿

• DOI: 10.1117/12.2509590
• 发表时间: 2019
• 作者: Thrapp A

Inline holographic microscopy through fiber imaging bundles

通过光纤成像束的在线全息显微镜

• DOI: 10.48550/arxiv.2006.09296
• 发表时间: 2020
• 作者: Hughes M

Towards high speed needle microscopy through a multimode fiber by single pixel imaging

• DOI: 10.1117/12.2509869
• 发表时间: 2019-02
• 作者: Chaitanya K. Mididoddi;M. Hughes