Structural Biology in the RFI

RFI 中的结构生物学

• 批准号: EP/S025243/1
• 负责人: James Naismith
• 金额: $ 210.18万
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FS025243%2F1
• 关键词: Structural; Biology; RFI

The RFI is a UK government funded initiative that will bring together Academia and Industry to solve the most pressing problems in the life sciences (Industrial Biotechnology, Pharmaceutical, Agrichemical). It has a Hub at Harwell and currently ten University partners (Spokes). Its goal is to deliver innovative new physical science that will transform the life sciences. The RFI has five interlinked scientific themes; structural biology, next generation chemistry, imaging with sound and light, mass spectrometry and next generation electron microscopy. Four broad areas of structural biology are identified where breakthroughs in the underlying methodology would be transformative. These are the extension to larger transient assemblies, the transformation of structure-guided drug discovery, reaching into the fourth (time) dimension and tackling unstructured proteins.In order to deliver impact in these areas, the RFI will undertake research in new approaches to manipulating cells and macromolecules, sample preparation; novel sample delivery particularly to electron microscopes / tomographs; new hardware for imaging; correlating imaging data from different sources; in developing a new pipelines for fragment-based drug discovery; and in combining imaging modalities to reach into the time domain. To realise these goals RFI requires a unique partnership between 10 Universities, the UK's central Facilities and most importantly UK Industry.

RFI是英国政府资助的一项倡议，将把学术界和工业界聚集在一起，解决生命科学(工业生物技术、制药、农业化学)中最紧迫的问题。它在哈维尔有一个中心，目前有十个大学合作伙伴(Spokes)。它的目标是提供创新的新物理科学，将改变生命科学。RFI有五个相互关联的科学主题：结构生物学、下一代化学、声光成像、质谱学和下一代电子显微镜。确定了结构生物学的四个广泛领域，在这些领域，基础方法论的突破将具有变革性。为了在这些领域产生影响，RFI将研究操纵细胞和大分子的新方法，样品制备；新的样品输送，特别是电子显微镜/断层摄影术；新的成像硬件；不同来源的成像数据的相互关联；开发基于片段的药物发现的新管道；以及结合成像方式进入时间域。为了实现这些目标，RFI需要10所大学、英国的中央设施以及最重要的英国工业之间的独特合作伙伴关系。

项目成果

Cryo-plasma FIB/SEM volume imaging of biological specimens.

生物标本的冷冻血浆FIB/SEM体积成像。

• DOI: 10.7554/elife.83623
• 发表时间: 2023-02-21
• 期刊: eLife
• 影响因子: 7.7
• 作者: Dumoux M;Glen T;Smith JLR;Ho EML;Perdigão LMA;Pennington A;Klumpe S;Yee NBY;Farmer DA;Lai PYA;Bowles W;Kelley R;Plitzko JM;Wu L;Basham M;Clare DK;Siebert CA;Darrow MC;Naismith JH;Grange M
• 通讯作者: Grange M

Structure-function studies of the C3/C5 epimerases and C4 reductases of the Campylobacter jejuni capsular heptose modification pathways.

• DOI: 10.1016/j.jbc.2021.100352
• 发表时间: 2021-01
• 期刊: The Journal of biological chemistry
• 作者: Barnawi H;Woodward L;Fava N;Roubakha M;Shaw SD;Kubinec C;Naismith JH;Creuzenet C
• 通讯作者: Creuzenet C

The structure-function relationship of oncogenic LMTK3.

• DOI: 10.1126/sciadv.abc3099
• 发表时间: 2020-11
• 期刊: Science advances
• 影响因子: 13.6
• 作者: Ditsiou A;Cilibrasi C;Simigdala N;Papakyriakou A;Milton-Harris L;Vella V;Nettleship JE;Lo JH;Soni S;Smbatyan G;Ntavelou P;Gagliano T;Iachini MC;Khurshid S;Simon T;Zhou L;Hassell-Hart S;Carter P;Pearl LH;Owen RL;Owens RJ;Roe SM;Chayen NE;Lenz HJ;Spencer J;Prodromou C;Klinakis A;Stebbing J;Giamas G
• 通讯作者: Giamas G

Uncovering the chemistry of C-C bond formation in C-nucleoside biosynthesis: crystal structure of a C-glycoside synthase/PRPP complex.

• DOI: 10.1039/d0cc02834g
• 发表时间: 2020-07-14
• 期刊: Chemical communications (Cambridge, England)
• 作者: Gao S;Radadiya A;Li W;Liu H;Zhu W;de Crécy-Lagard V;Richards NGJ;Naismith JH
• 通讯作者: Naismith JH

Engineering Thermostability in Artificial Metalloenzymes to Increase Catalytic Activity

• DOI: 10.1021/acscatal.0c05413
• 发表时间: 2021-03-08
• 期刊: ACS CATALYSIS
• 影响因子: 12.9
• 作者: Doble, Megan, V;Obrecht, Lorenz;Kamer, Paul C. J.
• 通讯作者: Kamer, Paul C. J.