CHARACTERIZATION AND REGULATION OF RELEASE OF ATRIAL NATRIURETIC PEPTIDES

心房钠尿肽释放的表征和调节

• 批准号: 3822976
• 负责人: R ESKAY
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3822976
• 关键词: alcoholism /alcohol abuse; atrium; calcium; cardiovascular function; cell membrane; chemical binding; epinephrine; ethanol; high performance liquid chromatography; hormone regulation /control mechanism; human subject; immunochemistry; neurotransmitter metabolism; peptide hormone biosynthesis; pituitary gland; radioimmunoassay; saluretic; stretch receptors; vasopressins

Cardiac atria contain a family of peptides, collectively termed atrial natriuretic peptides (ANPs), which are derived from a common precursor and possess intrinsic natriuretic, diuretic, vasorelaxant and endocrine modulatory effects. Furthermore, recent immunocytochemical and radioimmunoassay (RIA) distribution studies have localized ANPs to areas of the CNS that suggest the ANPs are involved in central cardiovascular regulatory events and in the release and/or synthesis of certain pituitary gland hormones. Additional support for the possible involvement of ANPs in regulating pituitary gland function was obtained with the demonstration that high-affinity, specific ANP binding sites are present on pituitary gland membranes. Characterization by reversed phase high-pressure liquid chromatography and quantification by RIA of rat plasma ANPs revealed the presence of at least two forms of ANP ranging from 24-28 amino acids. Additional studies revealed that volume loading or pressor agents (e.g. adrenaline, vasopressin) which cause atrial stretch result in a rapid increase in circulating ANPs. The acute administration of ethanol to rats in order to achieve blood ethanol levels in the range of .2-.25% did not alter plasma levels of ANPs; however, chronic exposure of rats to ethanol vapors for 7-14 days lowered plasma ANP levels. On the basis of results obtained in vitro from cultured adult rat atrial cardiocytes, it would appear that our observed enhancement of ANPs in vivo are indirectly mediated since a number of pressor agents did not alter ANP secretion in vitro. To date only drugs or experimental conditions which alter intracellular Ca++ levels appear to modulate ANP secretion in cultured atrial myocytes.

心脏心房包含一个肽家族，统称为心房肽。 利钠肽（ANP），其来源于共同的前体， 具有内源性利钠、利尿、血管舒张和内分泌 调节作用 此外，最近的免疫细胞化学和 放射免疫测定（RIA）分布研究已将ANP定位于 提示ANP参与中枢性心血管疾病的CNS 调节事件和某些垂体的释放和/或合成 腺体激素 为国家联络点可能参与 调节垂体功能的作用 高亲和力，特异性ANP结合位点存在于垂体上， 腺膜 通过反相高压液相色谱法表征， 大鼠血浆ANP的RIA定量显示至少存在 两种形式的ANP，范围为24-28个氨基酸。 其他研究 揭示了容量负荷或升压剂（例如肾上腺素， 血管加压素）引起心房牵张， 循环ANP。 对大鼠急性给予乙醇， 达到0.2 - 0.25%范围内的血液乙醇水平不会改变血浆 ANP水平;然而，大鼠长期暴露于乙醇蒸汽， 7-14 d降低血浆ANP水平。 根据研究结果， 体外培养的成年大鼠心房心肌细胞，似乎我们的 观察到的ANP在体内的增强是间接介导的，因为许多 升压药对ANP分泌无明显影响。 到目前为止，只有药物 或改变细胞内Ca++水平的实验条件似乎 调节培养的心房肌细胞的心钠素分泌。