Rapid, portable, and scalable Covid-19 antibody testing

快速、便携且可扩展的 Covid-19 抗体测试

• 批准号: EP/V02874X/1
• 负责人: Stefan Howorka
• 金额: $ 56.21万
• 依托单位: University College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FV02874X%2F1
• 关键词: Rapid; portable; scalable; Covid; 19

Determining antibody levels in humans is crucial for monitoring immunity against Covid-19 andtackling the national crisis. Antibody levels report about a previous infection and help decidewhether people can return to work or live with others without spreading the disease. To beeffective for national screening, antibody testing should deliver accurate results to individualsideally within minutes, and be portable as well as high-throughput. Existing techniques based onimmunosorbent assays do not deliver these benefits due to the need for multiple liquid handlingsteps, signal amplification, insufficient accuracy, or read-out with bulky optical equipment.This project will deliver fast, portable, high-throughput and accurate antibody sensing bypioneering step-changing sensor nanopores from the lead PI at University College LondonChemistry (UCLC), and by integrating them into memory-stick-sized on-the-market kits fromindustrial partner and biotech unicorn Oxford Nanopore Technologies (ONT). These MinIONanalysis kits have ushered in a revolution in portable DNA sequencing and are currently used forunravelling the Covid-19 sequence. The PI has a strong working relationship with the company andhas licensed sequencing pore technology which has been one key component to make the MinIONa success. In this project, the technology will be adapted with wider nanopores tailored for Covid-19 antibodies. The new sensor pores can be plugged into the existing MinION kits without theneed for redesigning the device, thereby ensuring production to scale. The devices will be clinicallytested and benchmarked by Co-PI and intensive care and monitoring specialist Prof. Mervyn Singerat UCL Medicine (UCLM). All project partners have previously successfully worked together in jointgrants, for publications, or via technology licensing contracts.

检测人体抗体水平对于监测对新冠肺炎的免疫力和应对国家危机至关重要。抗体水平报告了以前的感染情况，并有助于决定人们是否可以重返工作岗位或与他人生活在一起，而不会传播疾病。为了有效地进行全国筛查，抗体检测应在几分钟内向个人提供准确的结果，并便于携带和高通量。基于免疫吸附分析的现有技术无法提供这些好处，因为需要多次液体处理步骤、信号放大、精度不足或使用笨重的光学设备读出。该项目将提供快速、便携、高通量和准确的抗体检测，绕过伦敦大学化学学院(UCLC)领先PI的变步传感器纳米孔，并通过将它们集成到来自行业合作伙伴和生物技术独角兽公司牛津纳米孔技术公司(ONT)的记忆棒大小的市场试剂盒中。这些微型离子分析试剂盒在便携式DNA测序方面带来了一场革命，目前正用于解开新冠肺炎序列。PI与该公司有着牢固的工作关系，并获得了测序孔技术的许可，这是使MinIONa成功的关键组件之一。在这个项目中，这项技术将被改造成为新冠肺炎抗体量身定做的更宽的纳米孔。新的传感器气孔可以插入现有的Minion套件，而不需要重新设计设备，从而确保规模化生产。这些设备将由Co-Pi和UCL医学(UCLM)的重症监护和监测专家Mervyn Singerat教授进行临床测试和基准测试。所有项目伙伴以前都曾通过联合赠款、出版物或通过技术许可合同成功地合作。

项目成果

A reversibly gated protein-transporting membrane channel made of DNA.

• DOI: 10.1038/s41467-022-28522-2
• 发表时间: 2022-04-28
• 期刊: Nature communications
• 影响因子: 16.6

A Light-Triggered Synthetic Nanopore for Controlling Molecular Transport Across Biological Membranes

用于控制跨生物膜分子运输的光触发合成纳米孔

• DOI: 10.1002/ange.202210886
• 发表时间: 2022
• 期刊: Angewandte Chemie
• 作者: Offenbartl-Stiegert D

Functional Nanopores Enabled with DNA

DNA 实现功能性纳米孔

• DOI: 10.1002/ange.202303103
• 发表时间: 2023
• 期刊: Angewandte Chemie
• 作者: Xing Y

Sizing up DNA nanostructure assembly with native mass spectrometry and ion mobility.

• DOI: 10.1038/s41467-022-31029-5
• 发表时间: 2022-06-24
• 期刊: Nature communications
• 影响因子: 16.6

A Light-Triggered Synthetic Nanopore for Controlling Molecular Transport Across Biological Membranes.

• DOI: 10.1002/anie.202210886
• 发表时间: 2022-12-23
• 期刊: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
• 影响因子: 16.6
• 作者: Offenbartl-Stiegert, Daniel;Rottensteiner, Alexia;Dorey, Adam;Howorka, Stefan
• 通讯作者: Howorka, Stefan