Scalable Manufacturing of Nanostructured Bioassemblies for Low-Cost Portable Biosensors

人们对开发能够以低成本和大批量可靠和可重复地产生具有功能生物活性的纳米结构材料的制造方法的需求日益增长。这些技术在广泛的应用中是需要的，特别是在医疗诊断以及环境和食品监测方面。例如，用于可穿戴生物传感设备、柔性生物电子设备、功能隐形眼镜、智能屏幕和智能包装的响应材料。例如，具有适当检测灵敏度和选择性的生物活性纳米结构对于开发用于家庭和护理点诊断的低成本设备尤为重要。这项研究将开发一种使用三维(3D)打印在纸和塑料等柔性和廉价的衬底上大规模制造功能生物活性纳米结构的工艺。这一奖项将使一种新的生物传感设备的开发成为可能，这些设备使用方便、携带方便、价格低廉。研究成果将通过期刊和会议演示来传播，包括纳米制造设备的传感能力的动手演示，为高中教师举办的课程改进和专业发展研讨会，与行业合作，以及为学生提供创业培训。该项目将开发具有明确的光学和电子特性以及生物功能的可扩展的纳米生物活性材料的制造方法。总体目标是通过印刷从根本上了解杂化生物活性纳米结构的形成机理，并利用这些知识开发可扩展的生产方法，用于低成本的诊断应用。研究小组将专注于环保印刷油墨的工程设计，这些油墨具有特征粘度和组成，可保持功能纳米级特性和生物活性。这些纳米结构将整合生物识别、信号放大和检测能力，并将作为一体式生物传感设备发挥作用。将开发制造步骤和检测方案，以促进整个传感单元的自动打印并实现无试剂操作。该方法可以扩展到其他具有定制功能的杂化纳米结构和器件的合成。该方法将为使用低成本、多功能和可控的制造工艺原位组装杂化生物活性纳米结构开辟道路。

There is a growing demand for developing manufacturing processes that can reliably and reproducibly generate nanostructured materials with functional bioactive properties at low cost and in large quantities. These technologies are needed in a wide range of applications, especially in medical diagnostics and in environmental and food monitoring. Examples include responsive materials for wearable biosensing devices, flexible bioelectronics, functional contact lenses, smart screens and intelligent packaging. For example, bioactive nanostructures that have the appropriate detection sensitivity and selectivity are particularly important for the development of low cost devices for home and point-of-care diagnosis. This research will develop a process for large scale manufacturing of functional bioactive nanostructures on flexible and inexpensive substrates such as paper and plastic, using three-dimensional (3D) printing. This award will enable the development of a new class of biosensing devices that are easy to use, portable and inexpensive. Research outcomes will be disseminated through journals and conference presentations, including hands-on demonstrations of the sensing capabilities of the nanomanufactured devices, curriculum enhancement and professional development workshops for high school teachers, collaboration with industry, and providing entrepreneurial training for students.This project will develop scalable fabrication methods for nanobioactive materials with defined optical and electronic properties and biological functionality. The overall goal is to gain fundamental understanding of the formation mechanism of hybrid bioactive nanostructures by printing and to use this knowledge to develop scalable production methods for low cost diagnostic applications. The research team will focus on the engineering design of eco-friendly printing inks of characteristic viscosity and composition that maintain functional nanoscale properties and bioactivity. These nanostructures will integrate biorecognition, signal amplification and detection capabilities and will function as all-in-one biosensing devices. Fabrication steps and detection schemes will be developed to facilitate automatic printing of the entire sensing unit and enable reagentless operation. The approach can be expanded to the synthesis of other hybrid nanostructures and devices with tailored functionality. The method will open the way for the in situ assembly of hybrid bioactive nanostructures using a low cost, versatile and controllable manufacturing process.