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Nanoscale Building Blocks Based on Chemically Modified DNA Helices

基于化学修饰 DNA 螺旋的纳米级构建模块

基本信息

批准号：
EP/D030005/1
负责人：
Stefan Howorka
金额：
$ 15.62万
依托单位：
University College London
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2006
资助国家：
英国
起止时间：
2006 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=EP%2FD030005%2F1
关键词：
Nanoscale Building Blocks Based Chemically

项目摘要

This proposal is located at the interface between nanobiotechnology, chemistry and materials science and focuses on the generation of nanoscale building blocks and nanomechanical devices based on chemically modified DNA. The chemical modifications will help endow DNA with functional characteristics useful in material science and nanobiotechnology such as the ability to change the shape of the nanoscale building blocks in response to an external trigger. The target structure of the proposal is a DNA nanobarrel in which the wall of the barrel is either composed of stacks of DNA-circles or of a coil of an individual DNA duplex. The stacks of DNA-circles and the whorls of the individual DNA duplex will be held together by a multitude of tight yet reversible metal affinity complexes whose constituents are covalently attached to the chemically modified bases of the DNA. Two applications are envisioned for the DNA nanobarrels. (i) Nanobarrels can either serve as nanoscopic building blocks for the creation of lateral assemblies of microscale expansion. These can be employed as templates for the growth of inorganic or metallic nanorods or as nanofiltration membranes with controllable porosity using covalently attached stimulus-responsive polymers. (ii) Nanobarrels composed of a coil of an individual DNA strand can also be used as mechanical nanoscopic coil springs whose expansion is triggered by the controlled opening of the metal affinity bridges and fuelled by electrostatic repulsion between the whorls of the DNA nanobarrels. This type of nanomechanical device might be used as a nanoactuator to increase the distance between two objects or as a biosensor to detect heavy metal ions or toxins.

该提案位于纳米生物技术，化学和材料科学之间的接口，并专注于基于化学修饰的DNA生成纳米级构建块和纳米机械设备。化学修饰将有助于赋予DNA在材料科学和纳米生物技术中有用的功能特性，例如改变纳米级构建块形状以响应外部触发的能力。该提案的目标结构是一个DNA纳米桶，其中桶的壁由DNA环的堆叠或单个DNA双链体的线圈组成。DNA环的堆叠和单个DNA双链体的螺旋将通过大量紧密但可逆的金属亲和络合物保持在一起，所述金属亲和络合物的成分共价连接到DNA的化学修饰的碱基。设想了DNA纳米管的两种应用。(i)纳米管可以作为纳米级的构建块，用于创建微观尺度扩展的横向组件。这些可以用作无机或金属纳米棒生长的模板，或者用作使用共价连接的刺激响应性聚合物的具有可控孔隙率的纳滤膜。(ii)由单个DNA链的螺旋组成的纳米管也可以用作机械纳米级螺旋弹簧，其膨胀由金属亲和桥的受控打开触发，并由DNA纳米管的螺旋之间的静电排斥推动。这种类型的纳米机械装置可以用作纳米致动器来增加两个物体之间的距离，或者用作生物传感器来检测重金属离子或毒素。