3D Printing for World Class Additive Biofabrication

我们需要两件核心设备来增强我们的能力和世界级添加剂生物制造的能力。这些系统将允许存在生物细胞的3D打印。目前，在现有设施中，这是极具挑战性和高度不安全的-这些系统的采购将使联合国和英国成为该领域的世界领导者，并在国际竞争中领先一步。增材生物制造是使用增材制造（又名3D打印）来制造复杂的生物启发结构，在制造过程中存在细胞。这一点很重要，因为生物存在于3D中，当试图使用支架和类似结构替换、再生或治愈组织时，有细胞在场大大提高了成功的机会。拟议的技术对于支持未来的治疗至关重要，例如器官的恢复或再生以及新药的毒性筛选，但也准备为世界上一些最大的挑战提供工具和解决方案，包括通过应用于环境科学，生物技术和解决抗菌素耐药性来了解微生物如何与其生态系统相互作用。需要寻找三个项目。1：多光子打印机使3D打印能够在纳米尺度上发生，有可能实现小到100纳米的特征尺寸。我们需要一个环境保护的系统，允许在打印过程中使用生物细胞，这是诺丁汉目前不可能实现的一大步。2：微立体光刻印刷可在1-100微米范围内制造，提供互补的制造能力，但也允许更高的生产率，有可能在未来大规模生产。所要求的设备将构成增材生物制造中心的核心，补充诺丁汉大学资助的系统。用于微立体光刻系统的II级外壳，确保制造过程中的生物安全保护。关键的是，这些系统将允许用哺乳动物和微生物细胞制造。例如，这包括用于制造最终将导致治疗干预的组织的间充质干细胞和角膜干细胞，也包括可用于制造用于合成生物学目的的抗污染或促进收获的系统的细菌、真菌和藻类。所要求的物品将被安置在诺丁汉生物发现研究所的生物打印实验室。该环境是一个共享的实验室空间，致力于在哺乳动物和微生物细胞的制备和使用附近使用增材制造和相关技术。该实验室容纳了诺丁汉大学增材生物制造团队，该团队汇集了一组多学科和多部门的研究人员，他们对使用增材制造指导和控制细胞行为感兴趣和专业知识。这个小组包括工程、生命科学、再生医学和微生物学，旨在了解最终将导致的结构-功能关系，例如治疗干预或工业生物技术解决方案。该小组以再生医学和细胞疗法与诺丁汉大学增材制造中心（CfAM）之间的合作为中心，由寻求利用增材制造和再生医学之间接口的国际领先研究人员组成。

We request two pieces of core equipment to augment our capability and capacity for World Class Additive Biofabrication. These systems will allow for 3D printing with biological cells present. This is currently highly challenging and highly unsafe within current facilities - the procurement of these systems will allow UoN, and the UK, to become world leaders in this area and step ahead of the international competition. Additive biofabrication is the use of additive manufacturing (aka 3D printing) to manufacture complex, biologically inspired structures with cells present during the fabrication process. This is important because biology exists in 3D and when attempting to replace, regrow or heal tissues using scaffolds and similar structures, having cells present drastically improves the chances of success. The proposed technology is essential for underpinning future therapies, such as the restoration or regrowth of organs and in the screening of new medicines for toxicity, but is also poised to provide tools and solutions for some of the world's greatest challenges, including understanding how microbes interact with their ecosystem with application to environmental science, biotechnology and addressing antimicrobial resistance.Three items are sought.1: Multiphoton PrinterTo enable 3D printing to occur at the nanoscale, potentially realising feature sizes as small as 100 nm. We require a system with environmental protections that allow for the use of biological cells during printing, a major step forward that is not currently possible at Nottingham.2: Micro Stereolithography PrinterAble to fabricate in the 1-100 micron range, providing complementary manufacturing capability, but also allowing much higher production rates that potentially allow for mass manufacture in the future.The equipment requested will form the centrepiece of the Centre for Additive Biofabrication, complementing systems funded by the University of Nottingham. 3. A class II enclosure for the micro stereolithography system to ensure bio-safety protection during fabrication. Critically, these systems will allow for manufacture with mammalian and microbial cells. This includes for example, mesenchymal and corneal stem cells to create tissues that will ultimately lead to therapeutic interventions, but also bacteria, fungi and algae which can be used to produce systems that are either anti-fouling or promote harvesting for synthetic biology purposes. The requested items will be housed within the Bioprinting Laboratory in the Nottingham Biodiscovery Institute. This environment is a shared laboratory space dedicated to the use of additive manufacturing and related technologies in close proximity to the preparation and use of mammalian and microbial cells. This laboratory houses the University of Nottingham Additive Biofabrication team which brings together a group of multidisciplinary and multi-departmental researchers with an interest and expertise in using additive manufacturing for guiding and controlling cell behaviour. This grouping encompasses engineering, life sciences, regenerative medicine and microbiology and aims to understand the structure-function relationships that will eventually lead to, for example therapeutic interventions or industrial biotechnology solutions. The group is centred on a collaboration between the Regenerative Medicine and Cellular Therapies and the Centre for Additive Manufacturing (CfAM) at the University of Nottingham and is constituted of internationally leading researchers seeking to exploit the interface between additive manufacturing and regenerative medicine.