ENVIRONMENTAL REGULATION OF SYMPATHOADRENAL PHENOTYPIC EXPRESSION

这个项目的主要目的是为了更清楚地了解 两种神经活性物质的表达和调节 是由一个细胞群体表达的。细胞数量将会是 研究的是从神经脊派生出来的那些，在 尤其是交感神经肾上腺分支的表型 对周围神经系统的影响。大多数这些细胞 从神经峰前体区分化为神经元嗜铬细胞 合成“经典”神经递质的细胞 一种或多种神经肽。环境因素已被证明 以确定和调节基因的获取和表达 然而，人们对神经递质的特性知之甚少 影响特定神经肽共表达的因素 或关于它们对保持或可塑性的影响 神经递质/神经肽表型。 未分化神经脊和胚胎的培养 交感神经节和肾上腺将被用来测试 环境对初始表型表达的影响 辨析步调与已表达的调式 各自的特点。免疫细胞化学和 将使用神经化学技术来识别和量化 显示不同表型的细胞数量。放射免疫分析 将被用于量化神经肽的数量和 纳入放射性前体将被用作一项措施 神经递质的表达。 结果应该会导致对表达式的更好的理解 神经递质、神经肽和多肽激素以及 神经细胞的分化和可塑性过程 和内分泌系统。

The main aim of this project is to provide a clearer understanding of the expression and modulation of two neuroactive substances that are expressed by a cell population. The cell populations to be studied are those that derive from the neural crest, and in particular those phenotypes present in the sympatho adrenal branch of the peripheral nervous system. Most of these cells differentiate from neural crest precursors into neurons chromaffin cells that synthesize a "classical" neurotransmitter together with one or more neuropeptides. Environmental factors have been shown to determine and modulate the acquisition and expression of the neurotransmitter properties, however, little is known about the factors that affect the co-expression of a particular neuropeptide or about their influence on the maintenance or plasticity of the neurotransmitter/neuropeptide phenotype. Cultures of undifferentiated neural crest and of embryonic sympathetic ganglia and adrenal gland will be used to test the environmental influence on the phenotypic expression at the initial differentiation step and on the modulation of already expressed characteristics, respectively. Immunocytochemical and neurochemical techniques will be used to identify and quantify the number of cells showing different phenotypes. Radioimmunoassay will be used to quantify the amount of neuropeptides and incorporation of radioactive precursors will be used as a measure of neurotransmitter expression. The results should lead to a better understanding of the expression of neurotransmitters, neuropeptides and peptide hormones and of the processes of differentiation and plasticity in cells of the nervous and endocrine systems.