ENVIRONMENTAL REGULATION OF SYMPATHOADRENAL PHENOTYPIC EXPRESSION

交感肾上腺表型表达的环境调节

• 批准号: 3915725
• 负责人: JOSE E GARCIA-ARRARAS
• 金额: --
• 依托单位: UNIVERSITY OF PUERTO RICO RIO PIEDRAS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3915725
• 关键词: adrenal glands; cell differentiation; chromaffin cells; cytogenetics; endocrine gland /system; environment; gene expression; genetic regulation; histochemistry /cytochemistry; immunochemistry; neural crest; neurochemistry; neurons; neuropeptides; neurotransmitters; peptide hormone; radioimmunoassay; secretion; sympathetic nervous system

The main aim of this project is to provide a clearer understanding of the expression and modulation of two neuroactive substances that are expressed by a cell population. The cell populations to be studied are those that derive from the neural crest, and in particular those phenotypes present in the sympatho adrenal branch of the peripheral nervous system. Most of these cells differentiate from neural crest precursors into neurons chromaffin cells that synthesize a "classical" neurotransmitter together with one or more neuropeptides. Environmental factors have been shown to determine and modulate the acquisition and expression of the neurotransmitter properties, however, little is known about the factors that affect the co-expression of a particular neuropeptide or about their influence on the maintenance or plasticity of the neurotransmitter/neuropeptide phenotype. Cultures of undifferentiated neural crest and of embryonic sympathetic ganglia and adrenal gland will be used to test the environmental influence on the phenotypic expression at the initial differentiation step and on the modulation of already expressed characteristics, respectively. Immunocytochemical and neurochemical techniques will be used to identify and quantify the number of cells showing different phenotypes. Radioimmunoassay will be used to quantify the amount of neuropeptides and incorporation of radioactive precursors will be used as a measure of neurotransmitter expression. The results should lead to a better understanding of the expression of neurotransmitters, neuropeptides and peptide hormones and of the processes of differentiation and plasticity in cells of the nervous and endocrine systems.

这个项目的主要目的是提供一个更清晰的理解 两种神经活性物质的表达和调节， 是由细胞群表达的。 细胞群体将 研究的是那些来自神经嵴，并在 特别是存在于交感肾上腺分支中的那些表型 周围神经系统。 这些细胞中的大多数 从神经嵴前体分化为嗜铬神经元 细胞合成一种“经典”神经递质， 一种或多种神经肽。 环境因素已被证明 以确定和调节的收购和表达的 然而，神经递质的性质，很少有人知道， 影响特定神经肽共表达的因素 或它们对维持或可塑性的影响， 神经递质/神经肽表型。 未分化神经嵴和胚胎神经嵴的培养 交感神经节和肾上腺将用于测试 环境对表型表达的影响 分化步骤和对已经表达的 特点，分别。 免疫细胞化学和 神经化学技术将用于识别和量化 显示不同表型的细胞数量。 放射免疫 将用于定量神经肽的量， 放射性前体的掺入将被用作一种措施， 神经递质的表达。 结果应该会导致更好地理解表达 神经递质，神经肽和肽激素， 神经细胞的分化和可塑性过程 和内分泌系统。