Intracellular Controlled Radical Polymerizations

细胞内受控自由基聚合

• 批准号: EP/V047035/2
• 负责人: Nico Bruns
• 金额: $ 9.51万
• 依托单位: TU Darmstadt
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FV047035%2F2
• 关键词: Intracellular; Controlled; Radical; Polymerizations

The interface of synthetic biology and (bio)materials is a highly promising, yet underexplored field of research. It can play a key role in addressing one of the biggest questions to mankind, which is how life started and how non-living materials became living. If synthetic polymers are successfully integrated into living cells, it will become feasible to induce effects within cells by bio-orthogonal triggers, thereby, opening up new avenues to gain control over cells, i.e. over their development and behavior. The overarching goal of this project is to integrate biocatalytic controlled radical polymerizations (bioCRP) into living cells as a fundamentally new approach to interface biological systems with synthetic polymers and polymeric nanostructures. This project aims to achieve a fundamental understanding of what happens when amphiphilic block copolymers are synthesized and self-assembled directly within cells, and how this process can be steered to form nanostructures or semi-synthetic cell membranes. To this end, methods for intracellular bioCRP and polymerization-induced self-assembly will be developed and the intracellular self-assembly of block copolymers and their integration into the cell membrane will be studied.

合成生物学和（生物）材料的界面是一个非常有前途的，但未充分探索的研究领域。它可以在解决人类最大的问题之一，即生命如何开始以及非生命材料如何变得有生命方面发挥关键作用。如果合成聚合物成功整合到活细胞中，通过生物正交触发器在细胞内诱导效应将变得可行，从而开辟了控制细胞的新途径，即控制其发育和行为。该项目的总体目标是将生物催化控制自由基聚合（bioCRP）整合到活细胞中，作为一种全新的方法将生物系统与合成聚合物和聚合物纳米结构相结合。该项目旨在从根本上了解两亲性嵌段共聚物在细胞内直接合成和自组装时会发生什么，以及如何引导这一过程形成纳米结构或半合成细胞膜。为此，将开发用于细胞内bioCRP和聚合诱导的自组装的方法，并将研究嵌段共聚物的细胞内自组装及其整合到细胞膜中。

项目成果

Artificial cell synthesis using biocatalytic polymerization-induced self-assembly

• DOI: 10.1038/s41557-023-01391-y
• 发表时间: 2023-12-04
• 期刊: NATURE CHEMISTRY
• 影响因子: 21.8
• 作者: Belluati，Andrea;Jimaja，Setuhn;Bruns，Nico
• 通讯作者: Bruns，Nico

Synthesis of artificial cells via biocatalytic polymerisation-induced self-assembly

通过生物催化聚合诱导自组装合成人造细胞

• DOI: 10.21203/rs.3.rs-2889443/v1
• 发表时间: 2023
• 作者: Jimaja S

Self-decorating cells via surface-initiated enzymatic controlled radical polymerization.

通过表面引发的酶控制自由基聚合来自我装饰细胞。

• DOI: 10.1039/d3nr04008a
• 发表时间: 2023
• 期刊: Nanoscale
• 影响因子: 6.7
• 作者: Belluati A