3D Photoelectrochemical imaging in porous light-addressable structures

多孔光可寻址结构中的 3D 光电化学成像

• 批准号: EP/V047523/1
• 负责人: Steffi Krause
• 金额: $ 25.77万
• 依托单位: Queen Mary University of London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FV047523%2F1
• 关键词: 3D; Photoelectrochemical; imaging; porous; light

Electrochemical imaging techniques are powerful tools for the investigation of topography, charge and catalytic activity of surfaces and can also be used for functional, label-free imaging of cellular processes with high resolution. In electrochemical applications such as energy harvesting and storage devices, these techniques can be used to investigate local currents on complex electrode surfaces, thereby aiding the development of new and efficient electrode materials. In biology, electrochemical imaging has been used to carry out detailed investigations into the transport of molecules into or out of living cells, changes in cell morphology in response to stimulation and charges and action potential of cells. However, current electrochemical imaging technologies have been limited to probing thin films or the outer surfaces of materials or living cells. Recently, there has been a strong move towards 3D structures in both electrocatalysis and biology. Complex porous electrode structures have been developed for energy production and storage devices such as photocatalytic water splitting, redox flow batteries, fuel cells, batteries, supercapacitors and electrolysers. Detailed information about the local kinetics of electrochemical processes within pores of different shapes, sizes and interconnectivity and local effects of liquid and gas exchange during continuous operation could inform the development of novel electrode materials, but is not accessible to current electrochemical techniques. Cell biology has been swiftly moving from 2D cell culture to more complicated systems such as 3D tissue culture where complex physiological processes are investigated in an environment that resembles that of natural tissue, such as in organ-on-a chip devices, leaving the electrochemical imaging techniques behind to play catch-up. Only the outer surface of 3D structures and cells has been accessible to electrochemical scanning probe techniques, while photoelectrochemical techniques have been entirely limited to 2D imaging of flat substrates.In this project we aim to develop a photoelectrochemical imaging system that can be used for the mapping of electrochemical processes in three dimensions within porous electrode structures. The new technology is expected to aid the development of novel electrode materials for energy harvesting and storage devices and be suitable for in-situ 3D functional imaging in 3D tissue culture to contribute to the growing research area of organ-on-a chip devices, which aims to speed up the development of new treatment strategies and the discovery of new drugs for a vast range of diseases, while at the same time reducing the need for animal experiments.

电化学成像技术是研究表面的地形，电荷和催化活性的强大工具，也可用于对具有高分辨率的细胞过程的功能性，无标签成像。在电化学应用（例如能源收集和存储设备）中，这些技术可用于研究复杂电极表面上的局部电流，从而有助于开发新的有效的电极材料。在生物学中，电化学成像已被用来对分子进入或流出活细胞的运输，细胞形态的变化，响应细胞的刺激和电荷和动作潜力，对分子的运输进行了详细研究。但是，当前的电化学成像技术仅限于探测薄膜或材料或活细胞的外表面。最近，在电催化和生物学方面朝着3D结构方面有很大的进展。已经开发了复杂的多孔电极结构，用于能源生产和储存设备，例如光催化水分，氧化还原流量电池，燃料电池，电池，超级电容器和电解器。有关在连续运行期间液体和气体交换的毛孔，大小和互连性以及局部影响的毛孔，大小和互连性的局部动力学的详细信息可以为新型电极材料的开发提供信息，但对于当前的电化学技术无法访问。细胞生物学一直在迅速从2D细胞培养转变为更复杂的系统，例如3D组织培养物，在类似于天然组织的环境中，在类似于天然组织的环境中研究了复杂的生理过程，例如在A On-A-A芯片芯片设备中，将电化学成像技术留在后面。仅3D结构和细胞的外表面才能用于电化学扫描探针技术，而光电化学技术完全限于平坦底物的2D成像。在该项目中，我们旨在开发可用于在三二元组中的电化学性能映射的光电化学成像系统。预计这项新技术将有助于开发新型电极材料，以进行能源收集和存储设备，并适合在3D组织培养中的原位3D功能成像，从而有助于促进A芯片芯片设备的不断增长的研究领域，旨在加快新处理策略的开发，并在各种疾病中发现新药物，同时需要进行动物实验。

项目成果

Photoelectrochemical imaging of action potentials for single cardiomyocytes through contact force manipulation of organoids

• DOI: 10.1101/2022.09.05.506608
• 发表时间: 2022-09
• 期刊: bioRxiv
• 作者: Rachel Jacques;Bo Zhou;Emilie Marhuenda;Jon Gorecki;Anirban Das;T. Iskratsch;S. Krause

Photoelectrochemical detection of calcium ions based on hematite nanorod sensor substrates

基于赤铁矿纳米棒传感器基底的钙离子光电化学检测

• DOI: 10.26434/chemrxiv-2022-fhq71
• 发表时间: 2022
• 作者: Zhou B

Photoelectrochemical Detection of Calcium Ions Based on Hematite Nanorod Sensors.

• DOI: 10.1021/acsanm.2c03978
• 发表时间: 2022-11-25
• 期刊: ACS APPLIED NANO MATERIALS
• 影响因子: 5.9
• 作者: Zhou, Bo;Jiang, Yunlu;Guo, Qian;Das, Anirban;Sobrido, Ana Belen Jorge;Hing, Karin A.;V. Zayats, Anatoly;Krause, Steffi
• 通讯作者: Krause, Steffi