The York Physics of Pyrenoids Project (YP3): Nanostructured Biological LLPS:Next-Level-Complexity Physics of CO2-fixing Organelles

约克核糖体物理学项目 (YP3):纳米结构生物 LLPS:二氧化碳固定细胞器的新水平复杂性物理学

基本信息

  • 批准号:
    EP/W024063/1
  • 负责人:
  • 金额:
    $ 249.3万
  • 依托单位:
  • 依托单位国家:
    英国
  • 项目类别:
    Research Grant
  • 财政年份:
    2022
  • 资助国家:
    英国
  • 起止时间:
    2022 至 无数据
  • 项目状态:
    未结题

项目摘要

Single celled algae are among the most productive of all organisms on Earth for capturing carbon, fixing approximately 30% of all global CO2, yet we still do not understand all of the coupled processes by which they do it. Central to the mechanism are remarkable microscopic and self-assembled 'droplets' within the algae called 'Pyrenoids.' These 'condensates' within the cells are remarkable in their ability to form and dissolve as required, and unusual in not possessing a lipid membrane covering. They concentrate the proteins that need to interact to harvest and fix CO2 from the environment. Pyrenoids belong to a class of such separated droplets under intense study currently, but are more complex than other types as they also contain internal lipid membrane structures as tubes or layers, and some additionally generate starch platelets on their surface They seem to build on principles of 'Liquid-Liquid Phase Separation' well-studied in non-living systems with experimental and theoretical methods from physics.The York Pyrenoid Physics Project (YP3) will bring physicists and biologists together in an intensively collaborative team, to identify the key components and mechanisms of self-assembly, function and dis-assembly of the pyrenoid. The team combines expertise in algal molecular/cell biology, novel biophysical experimentation and imaging, and theoretical/computational biological physics. YP3 impacts the physics of biological assembly, algal and food-chain biotechnology as well as carbon capture. It will also represent a step-change in complexity in biological liquid-liquid phase separation (LLPS).The team will survey the structure and genetics of a large family of algal pyrenoids, then focus on intensive studies of core examples using cutting-edge imaging techniques able to track individual protein molecules as they move around the cell in response to external signals. Simplified mixtures of pyrenoid components, such as the active protein Rubsico and its 'linker' proteins, will be extracted from algae and the co-operative behaviour studied through the same techniques in vitro. The experiments will inform and test a growing computational model of the self-assembling pyrenoid, which will in turn make predictions for further experiments. A final output of the project will be the knowledge-base, from the combined model and experimental data, necessary to guide an attempt to build an artificial test tube pyrenoid, a next step in biotechnology applications for carbon capture and synthetic pyrenoids in crops to improve yields.The early-career researchers in YP3 will have unparalleled training in interdisciplinary research methods, communication and project management, and participate in wider Physics of Life activities in the UK and US through the UK Physics of Life and the linked US Physics of Living Systems Networks. YP3 is also supported by a world-leading international advisory board, who will additionally host the researchers for training periods.
单细胞藻类是地球上所有生物中最能捕捉碳的生物之一,固定了全球大约30%的二氧化碳,但我们仍然不知道它们是如何做到这一点的所有耦合过程。这一机制的核心是藻类中显著的微小和自组装的“液滴”,称为“蛋白核”。这些细胞内的“凝结物”在它们按要求形成和溶解的能力上是惊人的,而且不寻常的是没有脂质膜覆盖。它们集中了需要相互作用的蛋白质,以从环境中收集和固定二氧化碳。类蛋白核属于目前正在深入研究的一类分离的液滴,但比其他类型的液滴更复杂,因为它们还包含管状或层状的内部脂膜结构,并且一些类核素还在其表面生成淀粉小片,它们似乎建立在非生物系统中利用物理实验和理论方法很好地研究过的液-液相分离原理。约克类核素物理项目(YP3)将把物理学家和生物学家聚集在一个密切合作的团队中,以确定类核素的自组装、功能和分解的关键成分和机制。该团队结合了藻类分子/细胞生物学、新颖的生物物理实验和成像以及理论/计算生物物理学方面的专业知识。YP3影响生物组装、藻类和食物链生物技术以及碳捕获的物理学。这也将代表着生物液-液相分离(LLP)复杂性的阶段性变化。该团队将调查一大类藻类蛋白核的结构和遗传学,然后专注于使用尖端成像技术对核心实例进行深入研究,该技术能够跟踪单个蛋白质分子在细胞内对外部信号做出反应时的移动。我们将从藻类中提取类似蛋白核成分的简化混合物,如活性蛋白质Rubsico及其连接物蛋白质,并通过同样的技术在体外研究它们的合作行为。这些实验将提供并测试一个不断增长的自组装类蛋白核的计算模型,该模型将反过来为进一步的实验做出预测。该项目的最终成果将是从组合的模型和实验数据中获得必要的知识库,以指导建立人造试管蛋白核的尝试,这是生物技术应用于碳捕获和作物中合成蛋白核以提高产量的下一步。YP3的早期职业研究人员将在跨学科研究方法、沟通和项目管理方面接受无与伦比的培训,并通过英国生命物理和相连的美国生命系统物理网络参与英国和美国更广泛的生命物理活动。YP3还得到了世界领先的国际顾问委员会的支持,该委员会还将接待研究人员进行培训。

项目成果

期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Predicting Rubisco:Linker Condensation from Titration in the Dilute Phase
通过稀释相滴定预测 Rubisco:连接子缩合
  • DOI:
    10.48550/arxiv.2301.05681
  • 发表时间:
    2023
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Payne-Dwyer A
  • 通讯作者:
    Payne-Dwyer A
Specific isotopic labelling and reverse labelling for protein NMR spectroscopy: using metabolic precursors in sample preparation.
蛋白质NMR光谱的特定同位素标记和反向标记:在样品制备中使用代谢前体。
Community-developed checklists for publishing images and image analyses
社区开发的用于发布图像和图像分析的清单
  • DOI:
    10.1038/s41592-023-01987-9
  • 发表时间:
    2023
  • 期刊:
  • 影响因子:
    48
  • 作者:
    Schmied, Christopher;Nelson, Michael S.;Avilov, Sergiy;Bakker, Gert-Jan;Bertocchi, Cristina;Bischof, Johanna;Boehm, Ulrike;Brocher, Jan;Carvalho, Mariana T.;Chiritescu, Catalin
  • 通讯作者:
    Chiritescu, Catalin
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Mark Leake其他文献

Stoichiometry of Active DNA Replication Machinery Within Living Escherichia Coli Cells
  • DOI:
    10.1016/j.bpj.2009.12.3312
  • 发表时间:
    2010-01-01
  • 期刊:
  • 影响因子:
  • 作者:
    David Sherratt;Rodrigo Reyes-Lamothe;Mark Leake
  • 通讯作者:
    Mark Leake
Dynamics and Co-Localization of the Electron Transport Chain of Escherichia Coli: Investigations Through Fluorescence Microscopy
  • DOI:
    10.1016/j.bpj.2009.12.1265
  • 发表时间:
    2010-01-01
  • 期刊:
  • 影响因子:
  • 作者:
    Alex Robson;Mark Leake
  • 通讯作者:
    Mark Leake

Mark Leake的其他文献

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{{ truncateString('Mark Leake', 18)}}的其他基金

The Biophysics of Mesoscale, Reversible, Biomolecular Assemblies
中尺度可逆生物分子组装的生物物理学
  • 批准号:
    EP/Y000501/1
  • 财政年份:
    2024
  • 资助金额:
    $ 249.3万
  • 项目类别:
    Fellowship
How bacteria replicate their DNA in spite of barriers, one molecule at a time
细菌如何克服障碍,一次复制一个分子的 DNA
  • 批准号:
    BB/W000555/1
  • 财政年份:
    2021
  • 资助金额:
    $ 249.3万
  • 项目类别:
    Research Grant
Physics of Life Network+ (PoLNet3)
生命物理网络 (PoLNet3)
  • 批准号:
    EP/T022000/1
  • 财政年份:
    2020
  • 资助金额:
    $ 249.3万
  • 项目类别:
    Research Grant
Biological physics of protein clustering in epigenetic memory and transcriptional control
表观遗传记忆和转录控制中蛋白质聚类的生物物理学
  • 批准号:
    EP/T002166/1
  • 财政年份:
    2019
  • 资助金额:
    $ 249.3万
  • 项目类别:
    Research Grant
Tackling tricky twists - how does DNA gyrase function inside living cells?
解决棘手的问题——DNA 旋转酶在活细胞内如何发挥作用?
  • 批准号:
    BB/R001235/1
  • 财政年份:
    2018
  • 资助金额:
    $ 249.3万
  • 项目类别:
    Research Grant
Pushing proteins off DNA - how do helicases unwind protein-coated DNA?
将蛋白质从 DNA 上推开 - 解旋酶如何解开蛋白质包被的 DNA?
  • 批准号:
    BB/P000746/1
  • 财政年份:
    2017
  • 资助金额:
    $ 249.3万
  • 项目类别:
    Research Grant
Replication repair in real life: analysing how broken DNA replication machines are rebuilt inside cells.
现实生活中的复制修复:分析细胞内受损的 DNA 复制机器如何重建。
  • 批准号:
    BB/N006453/1
  • 财政年份:
    2016
  • 资助金额:
    $ 249.3万
  • 项目类别:
    Research Grant
Advanced multidimensional optics to investigate biological complexity at the single-molecule level in living, functional cells
先进的多维光学技术可在活的功能细胞的单分子水平上研究生物复杂性
  • 批准号:
    EP/G061009/1
  • 财政年份:
    2009
  • 资助金额:
    $ 249.3万
  • 项目类别:
    Research Grant

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