Phonon probe microscopy: towards acoustically-resolved Brillouin spectroscopy

声子探针显微镜：走向声分辨布里渊光谱

• 批准号: EP/W031876/1
• 负责人: Fernando Perez-Cota
• 金额: $ 51.6万
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FW031876%2F1
• 关键词: Phonon; probe; microscopy; towards; acoustically

Imaging living cells at super-optical resolution is a major breakthrough in microscopy which has enabled discovery in numerous areas of cell biology. However, the existing technologies present limitations. In particular, super-resolution based in fluorescence microscopy requires the use of dyes that are toxic to cells and can only be used for short periods. Electron microscopy is completely incompatible with live cells and atomic force microscopy is invasive and limited to the vicinity of the surface. Moreover, the characterisation of material properties with any of these techniques remains extremely challenging. To this day, the damage caused by short-wavelength radiation enforces strong restrictions to biological imaging. These limitations do not apply to phonons, which carry orders of magnitude less energy than electromagnetic radiation. This offers an exciting path for label-free, super-optical resolution microscopy. Moreover, imaging with phonons provides information related to the elasticity of the specimens. The combination of these aspects offers new possibilities in life sciences and healthcare applications. In this project, I propose a new way of achieving super-optical resolution by means of phonon acoustics. By using laser-generated phonon fields, it is possible to produce lenses small enough to produce tightly focused phonon beams with sub-optical wavelengths. The fields are to be detected by Brillouin scattering to provide elasticity-based contrast at safe optical wavelengths: proof of concept of the phonon-probe microscope.

以超光学分辨率成像活细胞是显微镜的一项重大突破，使细胞生物学的许多领域都有了新的发现。然而，现有的技术存在局限性。特别是，基于荧光显微镜的超分辨率需要使用对细胞有毒的染料，并且只能短期使用。电子显微镜与活细胞完全不相容，原子力显微镜是侵入性的，仅限于表面附近。此外，使用这些技术中的任何一种来表征材料特性仍然具有极大的挑战性。时至今日，短波辐射造成的损害对生物成像施加了强有力的限制。这些限制不适用于声子，因为声子携带的能量比电磁辐射少几个数量级。这为无标记、超光学分辨率显微镜提供了一条令人兴奋的途径。此外，声子成像提供了与样品弹性有关的信息。这些方面的结合为生命科学和医疗保健应用提供了新的可能性。在这个项目中，我提出了一种利用声子声学来实现超光学分辨率的新方法。通过使用激光产生的声子场，有可能制造出足够小的透镜，以产生具有亚光学波长的紧密聚焦的声子束。这些场将通过布里渊散射进行探测，以在安全的光学波长上提供基于弹性的对比度：声子探测显微镜的概念证明。