STABLE HEPATOCYTE CULTURES FOR IN VITRO CARCINOGENESIS

用于体外致癌作用的稳定肝细胞培养物

• 批准号: 2307780
• 负责人: FREDERICK CAHN
• 金额: $ 5万
• 依托单位: BIOMAT CORPORATION
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-06-18 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2307780
• 关键词: STABLE; HEPATOCYTE; CULTURES; VITRO; CARCINOGENESIS

Biomat's objective is to overcome the problems with hepatocyte cultures by developing biomaterials and culture media for long term cultures of organ- specific differentiated cells of human and animal origin capable of maintaining differentiated function to facilitate the development and validation of such tests. These materials together with assays of tissue specific function will be combined into test kits for toxicological studies. Stable extended culture allows for assay of tissue specific functions and the observations of events which may be correlated with carcinogenic action. Stable culture has important practical advantages due to the availability of cells from a single donor for many test campaigns and the ability to thoroughly characterize these cultures. Such practical advantages will be critical for commercialization of in vitro toxicological tests. Other advantages of cultures are observability and homogeneity. Our approach utilizes a collagen-glycosaminoglycan copolymer chemistry which allows us to construct stable materials configured as membranes, foams, coatings from purified components of the extracellular matrix.

Biomat的目标是通过以下方式克服肝细胞培养的问题： 开发用于长期培养器官的生物材料和培养基， 人类和动物来源的特异性分化细胞， 保持差异化功能，促进发展， 验证这些测试。 这些材料与组织分析一起 特定功能将被组合到毒理学检测试剂盒中， 问题研究 稳定的扩展培养允许测定组织特异性功能， 可能与致癌性相关的事件的观察结果 行动上 稳定的培养物具有重要的实际优势， 来自单个供体的细胞可用于许多测试活动， 有能力彻底描述这些文化。 这种实际 这些优势对于体外生物技术的商业化至关重要。 毒理学测试 文化的其他优势是可观察性， 同质性 我们的方法利用胶原蛋白-糖胺聚糖共聚物化学 这使我们能够构建稳定的材料， 泡沫、来自细胞外基质的纯化组分的涂层。

项目成果

The IGFI receptor gene: a molecular target for disrupted transcription factors.

IGFI 受体基因：被破坏的转录因子的分子靶标。

• DOI: 10.1002/gcc.10157
• 发表时间: 2003
• 期刊: Genes, chromosomes & cancer
• 作者: Werner,Haim;RobertsJr,CharlesT
• 通讯作者: RobertsJr,CharlesT