South West Regional Centre of Excellence for two-dimensional liquid chromatography with high resolution ion mobility mass spectrometry

西南地区二维液相色谱与高分辨率离子淌度质谱卓越中心

• 批准号: EP/W036444/1
• 负责人: Graham Langley
• 金额: $ 133.83万
• 依托单位: University of Southampton
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FW036444%2F1
• 关键词: South; West; Regional; Centre; Excellence

In its simplest terms mass spectrometry is a technique for weighing compounds or molecules. This is achieved through an initial stage where the neutral compound becomes charged. This is called ionisation, and once ionised, these species (ions) can be separated using magnetic and/or electric fields. For individual compounds analysis can be simple, but when dealing with mixtures other technologies are required. The mixtures need to be separated to identify the individual components; this is fundamentally separation science and there are several different ways to undertake this. The most common is called chromatography, which is a method of separating many different forms of chemical mixtures. The combination of chromatography and mass spectrometry affords the most powerful modern-day instrumentation for the analysis of complex mixtures.This proposal will fund a multiple inlets liquid chromatography mass spectrometer. This instrumentation will deliver advanced automated sample introduction techniques, separation of complex mixtures and advanced high resolution mass spectrometry to aid compound identification.The inlets will provide seamless integration and automated sample introduction with no constraints on compatible sample types.Liquid chromatography (one column) will afford separation of components in mixtures but for highly complex mixtures, 2-dimensional chromatography (two columns) are needed. The different columns enhance the separation of individual compounds and will separate of overlapping compounds. Ion mobility will provide another mode of separation based on shape, and finally high resolution mass measurement is used to determine the chemical formulae of compounds, this allows for species with the same nominal mass to be separated by differences in their chemical formulae.The combination of cutting-edge sample inlets, chromatographic separation and MS capability will deliver qualitative and quantitative analysis of novel and strategically important polar, thermally labile, involatile compounds in complex mixtures and matrices across a range of applications and will be a unique capability within the UK academic sector. The new strategic equipment will support research across the Southern region. The science from the regional partners (Universities of Bath, Portsmouth, Southampton, Surrey, and Swansea) will develop the capability and subsequently be extended to other academic and industry researchers to form a Centre of Excellence to enable high-priority EPSRC research.

用最简单的术语来说，质谱法是一种称量化合物或分子的技术。这是通过中性化合物带电的初始阶段实现的。这被称为电离，一旦电离，这些物质（离子）可以使用磁场和/或电场分离。对于单个化合物的分析可以很简单，但当处理混合物时，需要其他技术。混合物需要分离以识别单个组分;这基本上是分离科学，并且有几种不同的方法来进行。最常见的是色谱法，这是一种分离许多不同形式的化学混合物的方法。色谱和质谱的结合为复杂混合物的分析提供了最强大的现代仪器。本提案将资助多入口液相色谱质谱仪。该仪器将提供先进的自动进样技术、复杂混合物的分离和先进的高分辨率质谱分析，以帮助化合物鉴定。入口将提供无缝集成和自动进样，对兼容的样品类型没有限制。液相色谱（一个柱）将提供混合物中组分的分离，但对于高度复杂的混合物，需要二维色谱（两个柱）。不同的色谱柱增强了单个化合物的分离，并将分离重叠的化合物。离子迁移率将提供另一种基于形状的分离模式，最后使用高分辨率质量测量来确定化合物的化学式，这使得具有相同标称质量的物种可以通过其化学式的差异进行分离。尖端样品入口，色谱分离和MS功能的组合将提供对具有战略重要性的新型极性，在复杂的混合物和基质中的热不稳定，不挥发化合物的应用范围，将是英国学术界的一个独特的能力。新的战略设备将支持整个南部地区的研究。来自区域合作伙伴（巴斯大学、朴茨茅斯大学、南安普顿大学、萨里大学和斯旺西大学）的科学将发展这一能力，随后将扩展到其他学术和行业研究人员，形成一个卓越中心，以实现高优先级的EPSRC研究。