South West Regional Centre of Excellence for two-dimensional liquid chromatography with high resolution ion mobility mass spectrometry

用最简单的术语来说，质谱法是一种称量化合物或分子的技术。这是通过中性化合物带电的初始阶段实现的。这被称为电离，一旦电离，这些物种（离子）可以使用磁场和/或电场分离。对于单个化合物的分析可能很简单，但当处理混合物时，则需要其他技术。需要对混合物进行分离，以确定单个成分；这是基本的分离科学，有几种不同的方法来进行。最常见的是色谱法，这是一种分离许多不同形式的化学混合物的方法。色谱法和质谱法的结合为复杂混合物的分析提供了最强大的现代仪器。该提案将资助多入口液相色谱质谱计。该仪器将提供先进的自动化样品导入技术，分离复杂混合物和先进的高分辨率质谱，以帮助化合物鉴定。入口将提供无缝集成和自动示例导入，没有对兼容示例类型的限制。液相色谱法（一柱）可以分离混合物中的成分，但对于高度复杂的混合物，需要二维色谱法（两柱）。不同的柱加强了单个化合物的分离，并将重叠的化合物分离。离子迁移率将提供另一种基于形状的分离模式，最后使用高分辨率质量测量来确定化合物的化学式，这允许具有相同标称质量的物质通过其化学式的差异进行分离。尖端样品进样口、色谱分离和质谱能力的结合将为复杂混合物和基质中的新型和具有战略意义的极性、热不稳定、渐开式化合物提供定性和定量分析，并将成为英国学术界的独特能力。新的战略设备将支持整个南部地区的研究。来自区域合作伙伴（巴斯大学、朴茨茅斯大学、南安普顿大学、萨里大学和斯旺西大学）的科学将发展能力，并随后扩展到其他学术和行业研究人员，形成一个卓越中心，以实现EPSRC的高优先级研究。

In its simplest terms mass spectrometry is a technique for weighing compounds or molecules. This is achieved through an initial stage where the neutral compound becomes charged. This is called ionisation, and once ionised, these species (ions) can be separated using magnetic and/or electric fields. For individual compounds analysis can be simple, but when dealing with mixtures other technologies are required. The mixtures need to be separated to identify the individual components; this is fundamentally separation science and there are several different ways to undertake this. The most common is called chromatography, which is a method of separating many different forms of chemical mixtures. The combination of chromatography and mass spectrometry affords the most powerful modern-day instrumentation for the analysis of complex mixtures.This proposal will fund a multiple inlets liquid chromatography mass spectrometer. This instrumentation will deliver advanced automated sample introduction techniques, separation of complex mixtures and advanced high resolution mass spectrometry to aid compound identification.The inlets will provide seamless integration and automated sample introduction with no constraints on compatible sample types.Liquid chromatography (one column) will afford separation of components in mixtures but for highly complex mixtures, 2-dimensional chromatography (two columns) are needed. The different columns enhance the separation of individual compounds and will separate of overlapping compounds. Ion mobility will provide another mode of separation based on shape, and finally high resolution mass measurement is used to determine the chemical formulae of compounds, this allows for species with the same nominal mass to be separated by differences in their chemical formulae.The combination of cutting-edge sample inlets, chromatographic separation and MS capability will deliver qualitative and quantitative analysis of novel and strategically important polar, thermally labile, involatile compounds in complex mixtures and matrices across a range of applications and will be a unique capability within the UK academic sector. The new strategic equipment will support research across the Southern region. The science from the regional partners (Universities of Bath, Portsmouth, Southampton, Surrey, and Swansea) will develop the capability and subsequently be extended to other academic and industry researchers to form a Centre of Excellence to enable high-priority EPSRC research.