CORE--CAGED COMPOUND FACILITY

核心——笼式复合设施

• 批准号: 3736973
• 负责人: JEFFREY W WALKER
• 金额: --
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3736973
• 关键词: atomic absorption spectrometry; biomedical facility; chelating agents; chemical synthesis; diacylglycerols; inositol; mass spectrometry; nuclear magnetic resonance spectroscopy; optics; photochemistry; physical separation

The purpose of the this core facility is to provide photo-cleavable 'caged' compounds for use in subprojects within the Program. Compounds with known properties including caged chelators, caged pH probes, caged diacylglycerols and caged inositol phosphates will be made available in highly purified form. Several novel caged peptides, caged diacylglycerols and caged pH probes will be synthesized and characterized by chemical analysis, photolysis, and biochemical assays (enzyme or binding assays). In addition, chemical strategies will be explored for making certain compounds reversibly membrane permeant. The core will provide expertise in chemical synthesis, solid phase peptide synthesis, chemical analysis by nuclear magnetic resonance spectroscopy, UV/Vis absorption spectroscopy, mass spectroscopy, photochemistry and optics. Technical expertise in the use of caged compounds will also be provided including chromatographic analysis of purity, analysis of extents of photoconversion on photolysis, and optimization of photolysis optics for each experimental arrangement.

这个核心设施的目的是提供光可切割的“笼子”。