CORE--CAGED COMPOUND FACILITY

核心——笼式复合设施

• 批准号: 5213924
• 负责人: JEFFREY W WALKER
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5213924
• 关键词: atomic absorption spectrometry; biomedical facility; chelating agents; chemical synthesis; diacylglycerols; inositol; mass spectrometry; nuclear magnetic resonance spectroscopy; optics; photochemistry; physical separation

The purpose of the this core facility is to provide photo-cleavable 'caged' compounds for use in subprojects within the Program. Compounds with known properties including caged chelators, caged pH probes, caged diacylglycerols and caged inositol phosphates will be made available in highly purified form. Several novel caged peptides, caged diacylglycerols and caged pH probes will be synthesized and characterized by chemical analysis, photolysis, and biochemical assays (enzyme or binding assays). In addition, chemical strategies will be explored for making certain compounds reversibly membrane permeant. The core will provide expertise in chemical synthesis, solid phase peptide synthesis, chemical analysis by nuclear magnetic resonance spectroscopy, UV/Vis absorption spectroscopy, mass spectroscopy, photochemistry and optics. Technical expertise in the use of caged compounds will also be provided including chromatographic analysis of purity, analysis of extents of photoconversion on photolysis, and optimization of photolysis optics for each experimental arrangement.

该核心设施的目的是提供可光切割的“笼状” 用于该计划内子项目的化合物。 具有已知 特性包括笼状螯合剂、笼状pH探针、笼状 二酰基甘油和笼状肌醇磷酸将在 高度纯化的形式。 几种新型笼状肽、笼状甘油二酯 和笼状pH探针将合成和表征的化学 分析、光解和生物化学测定（酶或结合测定）。 此外，还将探讨化学策略， 化合物可逆膜渗透。 核心将提供以下方面的专门知识 化学合成，固相肽合成，化学分析， 核磁共振光谱法，UV/维斯吸收光谱法， 质谱、光化学和光学。 的技术专门知识 还将提供笼状化合物的使用， 纯度分析，光解光转化程度分析， 和优化每个实验装置的光解光学器件。