CORE--LIPID/PEPTIDE PROBE FACILITY

核心--脂质/肽探针设施

• 批准号: 6318387
• 负责人: JEFFREY W WALKER
• 金额: $ 19.96万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-10 至 2001-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6318387
• 关键词: analytical chemistry; biomedical facility; clathrate; diacylglycerols; fatty acids; fluorescent dye /probe; high performance liquid chromatography; mass spectrometry; nuclear magnetic resonance spectroscopy; peptide chemical synthesis; protein purification; radiotracer

Purpose This core will provide chemical synthesis services and expertise in probe development for subprojects within the Program. The majority of the service component will be developed to synthesis, purification, and analysis of peptides and caged compounds. Technical assistance in the use of commercial caged compounds will be provided including chromatographic analysis of compound purity, and determination of extent of photo-conversion under specific experimental conditions. Many of the compounds being used in the subprojects are not commercially available and mus be prepared on a regular basis. In some instances, compounds need to be radiolabeled, or have biotin or fluorophores attached for addressing specific issues regarding a compounds stability, localization, or effective concentration in cells. Labeling and the flexibility to otherwise customize reagents for specific purposes represent two of many significant advantages of having 'in house' chemical synthesis capabilities. Core staff in consultation with subproject personnel will design and implement synthetic strategies based upon the requirements of the physiological or biochemical system under investigation.

该中心将为该计划的子项目提供化学合成服务和探针开发方面的专业知识。大部分的服务组件将被开发为合成，纯化和分析肽和笼状化合物。将提供使用商业笼状化合物方面的技术援助，包括化合物纯度的色谱分析，以及确定在特定实验条件下的光转化程度。次级项目中使用的许多化合物在市场上买不到，必须定期制备。在一些情况下，化合物需要被放射性标记，或具有连接的生物素或荧光团以解决关于化合物在细胞中的稳定性、定位或有效浓度的特定问题。标记和为特定目的定制试剂的灵活性代表了具有“内部”化学合成能力的许多显著优点中的两个。核心工作人员将与子项目人员协商，根据所研究的生理或生化系统的要求设计和实施综合策略。