DYnamic control in hybrid plasmonic NAnopores: road to next generation multiplexed single MOlecule detection

混合等离子体纳米孔的动态控制：下一代多重单分子检测之路

• 批准号: EP/X038009/1
• 负责人: Ulrich Keyser
• 金额: $ 33.8万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FX038009%2F1
• 关键词: DYnamic; control; hybrid; plasmonic; NAnopores

In the quest to develop platforms to decipher the information in biomolecules there is an increasingly demand for techniques that are capable of identifying specific biomolecules at the smallest possible concentration, controlling their capturing and movement at the nanoscale. Nanopore sequencing is a key technology to drive this progress, and solid-state nanopores can be a major technological player. In the field of solid-state nanopores, many challenges remain in particular toward i) design and fabricate nanopore with size comparable to biological pores, ii) dynamic control of single molecule translocation, and iii) multiplexed read-out. Progress toward integrated platforms where both solid-state and biological nanostructures are implemented has been brought by the development of functional nanomaterials and DNA nanotechnology, able to address the importance of nanoscale precision in nanopore platforms with the potential to surpass the performance of biological nanopores. Yet, the integration of biological and synthetic nanomaterials is technically challenging, and their use to fully control and measure single molecule is far from real-world applications. DYNAMO is designed as an innovative research and training network, where we will recruit 10 Researchers to work toward the ambitious goal of developing the next-generation single molecule technologies exploiting hybrid DNA and metallic nanostructures. The network brings together a unique team of 8 world-leading academics and 1 high tech innovative company at the forefront of optical spectroscopy, DNA nanostructures, nanopore technology developments, single molecule detection and control, and biotech real-world applications. DYNAMO will establish an intersectoral training and research programme at the physics/chemistry/biotech interface with partners from 6 EU countries, aimed at creating the next generation of skilled well-connected scientists that will pioneer the 'single molecule science of tomorrow'.

在寻求开发平台来破译生物分子中的信息的过程中，对能够以尽可能小的浓度识别特定生物分子、在纳米级控制它们的捕获和移动的技术的需求越来越大。纳米孔测序是推动这一进步的关键技术，而固态纳米孔可能是主要的技术参与者。在固态纳米孔领域，仍然存在许多挑战，特别是在i)设计和制造与生物孔大小相当的纳米孔，ii)单分子移位的动态控制，以及iii)多路读出。功能纳米材料和DNA纳米技术的发展带来了实现固态和生物纳米结构的集成平台的进展，能够解决纳米孔平台中纳米级精度的重要性，并有可能超过生物纳米孔的性能。然而，生物和合成纳米材料的集成在技术上具有挑战性，使用它们来完全控制和测量单个分子还远远谈不上真正的应用。迪纳摩是一个创新的研究和培训网络，我们将在那里招募10名研究人员，朝着利用混合DNA和金属纳米结构开发下一代单分子技术的雄心勃勃的目标努力。该网络汇集了由8名世界领先的学者和1家高科技创新公司组成的独特团队，这些公司处于光学光谱、DNA纳米结构、纳米孔技术开发、单分子检测和控制以及生物技术现实应用的前沿。迪纳摩将与来自6个欧盟国家的合作伙伴一起，在物理/化学/生物技术接口上建立一个跨部门的培训和研究计划，旨在培养下一代熟练的、人脉广泛的科学家，他们将开创“明天的单分子科学”。