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1989年，巨细胞肝炎的一种可能的副粘病毒病因是 提出，根据电子显微镜观察到的肝脏变化， 患有这种疾病的患者。 合作研究，包括尝试 将疾病传染给灵长类动物的方法正在进行中。 意大利撒丁岛的地中海贫血患者每月接受血液 输血作为治疗。 这些患者中的许多人患有慢性 肝炎 大多数病例可被鉴定为B型肝炎或肝炎 C，但有些人缺乏这两种病毒的标记。 这些患者 正在研究以前未被发现的肝炎的证据 病毒 类似地，来自患有急性或慢性炎症的患者的临床样品， 美国的非甲、B、C、D、E型肝炎将被研究 传染源的生物学、血清学或分子学证据。 暴发性非甲、非乙型肝炎患者一般不会有 感染任何公认的肝炎病毒的证据。 只有约 10%可以被确定为患有暴发性丙型肝炎。 剩下的90% 仍然是一个诊断上的谜，可能感染了一种或多种 以前不认识的病毒 在合作研究中，我们 试图将疾病传染给灵长类动物 存在另一种水源性肝炎病毒的证据 来自于对肠道传播的非甲型流感病毒的血清流行病学研究， 印度的非乙型肝炎 16个国家50-100%的肝炎病例 水传播性肝炎的流行是由戊型肝炎病毒引起的，但水传播性肝炎的流行是由戊型肝炎病毒引起的。 流行既不是由HAV引起的，也不是由HEV引起的。 一种肝炎病毒 几年前报告说，它可以从非甲非乙型肝炎传播， 对绒猴的耐心。 该代理，称为GB代理，可能是 在绒猴中连续传播，并部分表征。 然而，血清学测试无法开发， 该病毒从未得到令人满意的测定。 此前的研究 实验室与GB代理正在重新启动，并在合作 研究，克隆病毒基因组正在尝试。 该项目的目标是确定和描述新的 肝炎的病原体，并开发有用的诊断方法 感染和血清流行病学研究。 长期目标是 被动和主动免疫预防的发展， 重要的人类病原体

In 1989 a possible paramyxovirus etiology for giant cell hepatitis was proposed, based on electron micrographic changes seen in the livers of patients with this disease. Collaborative studies, including attempts to transmit the disease to primates, are in progress. Patients with thalassemia in Sardinia, Italy, receive monthly blood transfusions as therapy. Many of these patients have developed chronic hepatitis. Most cases could be identified as hepatitis B or hepatitis C, but some lacked markers of either of these viruses. These patients are being studied for evidence of previously unrecognized hepatitis viruses. Similarly, clinical samples from patients with acute or chronic non-A,B,C,D,E hepatitis in the United States are to be studied for biological, serological or molecular evidence of transmissible agents. Patients with fulminant non-A, non-B hepatitis generally do not have evidence of infection with any recognized hepatitis virus. Only about 10% can be identified as having fulminant hepatitis C. The remaining 90% remain a diagnostic enigma and may be infected with one or more previously unrecognized viruses. In collaborative studies, we are attempting to transmit the disease to primates. Evidence for the existence of an additional water-borne hepatitis virus has come from seroepidemiologic studies of enterically transmitted non-A, non-B hepatitis in India. From 50-100% of hepatitis cases in 16 epidemics of water-borne hepatitis were caused by HEV but 1 water-borne epidemic was caused by neither HAV nor HEV. A hepatitis virus was reported some years ago to be transmissible from a non-A, non-B hepatitis patient to marmoset monkeys. The agent, called the GB agent, could be serially transmitted in marmosets and was partially characterized. However, serologic tests could not be developed and the true origin of the virus was never satisfactorily determined. Previous studies in this laboratory with the GB agent are being reactivated and, in collaborative studies, cloning of the viral genome is being attempted. The objectives of this project are to identify and characterize new etiologic agents of hepatitis and to develop useful assays for diagnosis of infection and seroepidemiologic studies. A longer term objective is the development of passive and active immunoprophylaxis for these important human pathogens.