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NEW APPROACHES TO THE IN VITRO PROPAGATION OF NON-CULTIVATABLE AGENTS

NEW APPROACHES TO THE IN VITRO PROPAGATION OF NON-CULTIVATABLE AGENTS
不可培养因子体外繁殖的新方法
批准号：
4688466
负责人：
R H PURCELL
金额：
$0.0万
依托单位：
NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/4688466
关键词：
hepatitis virus hepatocellular carcinoma hybridomas liver cells microorganism culture tissue /cell culture virus replication

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中文摘要
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英文摘要
There is a need for an in vitro substrate for the cultivation of hepatitis viruses. Hepatocytes would seem a logical choice, but it is very difficult to obtain and maintain primate hepatocytes in culture. We are attempting to develop heptocyte-hepatoma hybridomas of primate origin. Such hybrid cells would be expected to have the receptor sites and metabolic systems suitable for synthesis of hepatitis viruses and the ability of hepatoma cells to multiply indefinitely in vitro. Methods for the selection of hybrid cells without drug markers (i.e. use of vital dyes) have been developed.
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STUDIES OF ACQUIRED IMMUNE DEFICIENCY SYNDROME
SECOND AND THIRD-GENERATION HEPATITIS B VACCINES
WOODCHUCK HEPATITIS VIRUS
SECOND AND THIRD-GENERATION HEPATITIS B VACCINES
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