USE OF THE SERUM SEPARATOR FOR RAPID IDENTIFICATION OF BACTERIA IN BLOOD CULTURES

血培养呈阳性的报告是一个重大发现 尽快转告给医生。为了确保 选择适当的抗生素覆盖范围，重要的是 提供有关存在的生物体类型的信息。当 微生物在血液培养瓶中生长，它必须首先 继代培养到平板上，以获得可用于 鉴定和药敏试验。这通常会导致 一整晚的延误。直接接种鉴定试纸或 其他使用瓶子生长的测试不能可靠地进行，因为 可能没有足够数量的有机体或存在 血液可能会影响结果的准确性。我们发现， 通常用于从血液中提取血清的血清分离管， 可用于制备无血细胞的生物体颗粒 阳性血培养瓶。颗粒含有6-8个折叠层 生物体浓度，不会造成红细胞污染，因此 可以进行快速检测，以帮助确定属或 在肉汤培养的同一天的生物物种 阳性。这些研究定义了使用血清进行测试的准确性- 分离器颗粒。

The report of a positive blood culture Is a significant finding that is relayed to the doctor as soon as possible. in order to ensure that appropriate antibiotic coverage is selected, it is important to provide information as to the type of organism present. When the organism Is growing in a blood culture bottle, it must first be subcultured onto plates to obtain growth that can be used for identification and susceptibility testing. This frequently results in an overnight delay. Direct inoculation of Identification strips or other tests using growth from the bottle cannot be done reliably since there may be insufficient quantities of organism or the presence of blood may Interfere with accuracy of the result. We have found that the serum separator tube, normally used to prepare serum from blood, can be used to prepare a blood cell-free pellet of organism from positive blood culture bottles. The pellet contains a 6-8 fold concentration of organism, without red blood cell contamination, so that rapid tests can be performed to help determine the genus or species of organism on the same day that the broth culture is positive. These studies define the accuracy of tests using the serum- separator pellet.