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OPTIMAL METHODOLOGY FOR DETECTION OF HSV IN CLINICAL LABORATORY

OPTIMAL METHODOLOGY FOR DETECTION OF HSV IN CLINICAL LABORATORY
临床实验室检测 HSV 的最佳方法
批准号：
3874369
负责人：
V J GILL
金额：
$0.0万
依托单位：
CLINICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3874369
关键词：
Alphaherpesvirinae Herpes simplex disease communicable disease diagnosis diagnosis design /evaluation herpes simplex virus 1 herpes simplex virus 2 human tissue immunofluorescence technique immunologic assay /test monoclonal antibody virus classification

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中文摘要
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英文摘要
Two monoclonal fluorescent antibody kits were compared to a direct immunoperoxidase (DIP) stain being used routinely for staining shell vial preparations for Herpes simplex virus. Of 68 specimens tested in triplicate, 24 were positive for HSV. The Syva FA kit showed 100% sensitivity and 100% specificity when compared to the DIP results. The Bartels FA kit was slightly less sensitive (92%). The Syva kit was also brighter and easier to read than the Bartels. Either FA procedure was less labor intensive than the DIP, and also less expensive. For all of these reasons, this study resulted in the substitution of the Syva FA for the Bartels DIP for detection of HSV, and the project has been completed.
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  • 批准号：
    5201185
  • 项目类别：
  • 资助金额：
    $0.0万
  • 财政年份：
    --
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  • 依托单位：
    CLINICAL CENTER
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  • 批准号：
    4692052
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    $0.0万
  • 财政年份：
    --
  • 负责人：
    V J GILL
  • 依托单位：
    CLINICAL CENTER
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  • 批准号：
    4692062
  • 项目类别：
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    $0.0万
  • 财政年份：
    --
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  • 依托单位：
    CLINICAL CENTER
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  • 批准号：
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  • 项目类别：
  • 资助金额：
    $0.0万
  • 财政年份：
    --
  • 负责人：
    V J GILL
  • 依托单位：
    CLINICAL CENTER
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